Immunohistochemistry (IHC)
(Immunochemical staining of human RRAGB in human brain with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
Rabbit anti-Human RRAGB Polyclonal Antibody | anti-RRAGB antibody
Anti-RRAGB Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
Protein A & Antigen Affinity
Synonyms
RRAGB; Polyclonal Antibody; Anti-RRAGB Antibody; Rabbit Polyclonal; RRAGB Antibody; Rabbit PAb; Antigen Affinity Purified; Ras-related GTP binding B; Anti-bA465E19.1 Antibody; Anti-RAGB Antibody; anti-RRAGB antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human RRAGB
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-RRAGB antibody
Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
IHC-P: 1:200-1:1000
ICC/IF: 1:100-1:500
ICC/IF: 1:100-1:500
Immunogen
E Coli-derived Human RRAGB fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human RRAGB fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of human RRAGB in human brain with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human RRAGB in human brain with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
Immunofluorescence (IF)
(Immunofluorescence staining of RRAGB in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human RRAGB polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
Immunofluorescence (IF)
(Immunofluorescence staining of RRAGB in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human RRAGB polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
Related Product Information for anti-RRAGB antibody
RRAGB (Ras Related GTP Binding B) is a Protein Coding gene. The RRAGB gene, located on Xp11.21, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, fruit fly, mosquito, and C.elegans. All GTPases of the Ras superfamily have in common the presence of six conserved motifs involved in GTP/GDP binding, three of which are phosphate-/magnesium-binding sites (PM1-PM3) and three of which are guanine nucleotide-binding sites (G1-G3). Transcript variants encoding distinct isoforms have been identified. RRAGB is widely expressed in the brain, thyroid, and other tissues. Diseases associated with RRAGB include Polycystic Echinococcosis and Cystic Echinococcosis. Among its related pathways are Signaling by GPCR and RET signaling.
References
Schurmann A, et al. (1995) Cloning of a novel family of mammalian gtp-binding proteins (raga, ragbs, ragb1) with remote similarity to the ras-related gtpases. J Biol Chem 270 (48): 28982-28988.Hirose E, et al. (1998) Raga is a functional homologue of s. Cerevisiae gtr1p involved in the ran/gsp1-gtpase pathway. J Cell Sci 111 (Pt 1) 11-21.Duran RV, et al. (2012) Glutaminolysis activates rag-mtorc1 signaling. Mol Cell 47 (3): 349-358.
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Product Notes
The RRAGB (Catalog #AAA8109419) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RRAGB Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RRAGB can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF). IHC-P: 1:200-1:1000 ICC/IF: 1:100-1:500. Researchers should empirically determine the suitability of the RRAGB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RRAGB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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