Western Blot (WB)
(All lanes: Sulfotransferase family cytosolic 2B member 1 antibody at 4ug/ml+MCF-7 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 42,40 kDaObserved band size: 41 kDa)
Rabbit anti-Human Sulfotransferase family cytosolic 2B member 1 Polyclonal Antibody | anti-SULT2B1 antibody
Rabbit anti-human Sulfotransferase family cytosolic 2B member 1 polyclonal Antibody(SULT2B1)
IHC:1:20-1:200
Western Blot (WB)
(All lanes: Sulfotransferase family cytosolic 2B member 1 antibody at 4ug/ml+MCF-7 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 42,40 kDaObserved band size: 41 kDa)
Western Blot (WB)
(All lanes: Sulfotransferase family cytosolic 2B member 1 antibody at 4ug/ml+MCF-7 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 42,40 kDaObserved band size: 41 kDa)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human prostate using MBS1496676 at dilution 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human prostate using MBS1496676 at dilution 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human placenta tissue using MBS1496676 at dilution 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human placenta tissue using MBS1496676 at dilution 1:100)
Immunoprecipitation (IP)
(Immunoprecipitating SULT2B1 in MCF-7 whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of MBS1496676 in MCF-7 whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: MBS1496676(4ug)+ MCF-7 whole cell lysate(500ug)Lane 3: MCF-7 whole cell lysate (20ug))
Immunoprecipitation (IP)
(Immunoprecipitating SULT2B1 in MCF-7 whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of MBS1496676 in MCF-7 whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: MBS1496676(4ug)+ MCF-7 whole cell lysate(500ug)Lane 3: MCF-7 whole cell lysate (20ug))
NCBI and Uniprot Product Information
NCBI Description
Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene sulfates dehydroepiandrosterone but not 4-nitrophenol, a typical substrate for the phenol and estrogen sulfotransferase subfamilies. Two alternatively spliced variants that encode different isoforms have been described. [provided by RefSeq, Jul 2008]
Uniprot Description
SULT2B1: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs and xenobiotic compounds. Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. Sulfates hydroxysteroids like DHEA. Isoform 1 preferentially sulfonates cholesterol, and isoform 2 avidly sulfonates pregnenolone but not cholesterol. Belongs to the sulfotransferase 1 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Lipid Metabolism - androgen and estrogen; EC 2.8.2.2; Transferase; Energy Metabolism - sulfur
Chromosomal Location of Human Ortholog: 19q13.3
Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; intracellular membrane-bound organelle; nucleus
Molecular Function: alcohol sulfotransferase activity; protein binding; steroid sulfotransferase activity
Biological Process: 3'-phosphoadenosine 5'-phosphosulfate metabolic process; steroid metabolic process; sulfate assimilation; xenobiotic metabolic process
Research Articles on SULT2B1
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Product Notes
The SULT2B1 sult2b1 (Catalog #AAA1496676) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Sulfotransferase family cytosolic 2B member 1 polyclonal Antibody(SULT2B1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Sulfotransferase family cytosolic 2B member 1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP). Recommended dilution: WB:1:500-2000 IHC:1:20-1:200. Researchers should empirically determine the suitability of the SULT2B1 sult2b1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sulfotransferase family cytosolic 2B member 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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