Goat NPR3 Polyclonal Antibody | anti-NPR3 antibody

Goat Polyclonal to Human NPR3

Cat. Num. AAA248162

• Gene Names: NPR3; NPRC; ANP-C; ANPRC; NPR-C; ANPR-C; GUCY2B; C5orf23
• Reactivity: Gibbon, Bovine, Chimpanzee, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Orangutan, Rabbit, Rat; Predicted Reactivity: Bat, Chicken, Xenopus (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: NPR3; Polyclonal Antibody; Goat Polyclonal to Human NPR3; Anti-NPR3 Antibody (aa520-533) IHC-plus; ANPRC; C5orf23; GUCY2B; NPR-C; NPRC; ANP-C; ANPR-C; Human NPR3; anti-NPR3 antibody

• Host: Goat
• Reactivity: Gibbon, Bovine, Chimpanzee, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Orangutan, Rabbit, Rat; Predicted Reactivity: Bat, Chicken, Xenopus (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human NPR3. The immunizing peptide represents the cytoplasmic part of the protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 540

• Applicable Applications for anti-NPR3 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:16000), IHC-P (10 ug/ml), WB (1 - 3 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 52kD band observed in Human Placenta lysates (calculated MW of 59.8kD according to NP_000899.1). Recommended concentration: 1-3 ug/ml.Tested in IHC-P only with human FFPE...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-HRELREDSIRSHFS from the C-terminus of human NPR3 (NP_000899.1).
• Immunogen Type: Synthetic peptide
• Immunogen: NPR3 antibody was raised against synthetic peptide C-HRELREDSIRSHFS from the C-terminus of human NPR3 (NP_000899.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Horse, Rabbit, Porcine, Opossum (100%); Bat, Turkey, Zebra finch, Chicken, Xenopus (93%); Pufferfish, Zebrafish (86%).
• Antigen Modification: aa520-533
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-NPR3 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Western Blot (WB)

(NPR3 antibody (1 ug/ml) staining of Human Placenta lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 4505441
• NCBI GeneID: 4883
• NCBI Accession #: NP_000899.1
• NCBI GenBank Nucleotide #: NM_000908.3
• UniProt Accession #: P17342; A2RRD1; B4DT84; E7EPG9
• Molecular Weight: 36,656 Da
• NCBI Official Full Name: atrial natriuretic peptide receptor 3 isoform 2
• NCBI Official Synonym Full Names: natriuretic peptide receptor 3
• NCBI Official Symbol: NPR3
• NCBI Official Synonym Symbols: NPRC; ANP-C; ANPRC; NPR-C; ANPR-C; GUCY2B; C5orf23
• NCBI Protein Information: atrial natriuretic peptide receptor 3; guanylate cyclase C; atrionatriuretic peptide receptor C; atrial natriuretic peptide receptor type C; atrial natriuretic peptide clearance receptor; natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptide receptor C)
• UniProt Protein Name: Atrial natriuretic peptide receptor 3
• Protein Family: Nitrogen permease regulator
• UniProt Gene Name: NPR3
• UniProt Synonym Gene Names: ANPRC; C5orf23; NPRC; ANP-C; ANPR-C; NPR-C
• UniProt Entry Name: ANPRC_HUMAN

NCBI Description

This gene encodes one of three natriuretic peptide receptors. Natriutetic peptides are small peptides which regulate blood volume and pressure, pulmonary hypertension, and cardiac function as well as some metabolic and growth processes. The product of this gene encodes a natriuretic peptide receptor responsible for clearing circulating and extracellular natriuretic peptides through endocytosis of the receptor. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011]

Uniprot Description

ANP-C: Receptor for the natriuretic peptide hormones, binding with similar affinities atrial natriuretic peptide NPPA/ANP, brain natriuretic peptide NPPB/BNP, and C-type natriuretic peptide NPPC/CNP. May function as a clearance receptor for NPPA, NPPB and NPPC, regulating their local concentrations and effects. May regulate diuresis, blood pressure and skeletal development. Does not have guanylate cyclase activity. Belongs to the ANF receptor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5p13.3

Cellular Component: integral to plasma membrane

Molecular Function: protein homodimerization activity; hormone binding; natriuretic peptide receptor activity; peptide hormone binding; peptide receptor activity, G-protein coupled

Biological Process: regulation of osteoblast proliferation; phosphoinositide-mediated signaling; negative regulation of smooth muscle cell proliferation; regulation of blood pressure; positive regulation of nitric-oxide synthase activity; negative regulation of adenylate cyclase activity; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); G-protein signaling, adenylate cyclase inhibiting pathway; pancreatic juice secretion; skeletal development