Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with Bcl-2 BH3 Domain Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Rabbit anti-Human Bcl-2 Polyclonal Antibody | anti-Bcl-2 antibody
Bcl-2, BH3 Domain Specific (BCL2, Apoptosis regulator Bcl-2) (PE)
FLISA: 1:1,000
IHC: 1:10-50
IP: 1:100-500
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with Bcl-2 BH3 Domain Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with Bcl-2 BH3 Domain Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of Bcl-2 Antibody (BH3 Domain Specific) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of Bcl-2 Antibody (BH3 Domain Specific) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunoprecipitation (IP)
(Left panel, analysis of Bcl-2 BH3 domain exposure in HEK293 cells transfected with a plasmid coding for a DNA-binding domain-deleted construct of Nur77 (GFP-Nur77/dDBD) by using AP1303a antibody for immunoprecipitation (IP) and a different Bcl-2 antibody for western blot. Right panel, same analysis using AP1303a in flow cytometry. Data courtesy Dr Quan Chen, Chinese Acadamy of Sciences.)
Immunoprecipitation (IP)
(Left panel, analysis of Bcl-2 BH3 domain exposure in HEK293 cells transfected with a plasmid coding for a DNA-binding domain-deleted construct of Nur77 (GFP-Nur77/dDBD) by using AP1303a antibody for immunoprecipitation (IP) and a different Bcl-2 antibody for western blot. Right panel, same analysis using AP1303a in flow cytometry. Data courtesy Dr Quan Chen, Chinese Acadamy of Sciences.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq, Jul 2008]
Uniprot Description
Bcl-2: a antiapoptotic member of the Bcl-2 family. Regulates cell death by controlling the mitochondrial membrane permeability. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Phosphorylation by JNKs may increase its antiapoptotic functions.
Protein type: Membrane protein, integral; Oncoprotein; Autophagy; Apoptosis
Chromosomal Location of Human Ortholog: 18q21.3
Cellular Component: pore complex; endoplasmic reticulum membrane; mitochondrial outer membrane; nuclear membrane; membrane; mitochondrion; endoplasmic reticulum; cytoplasm; nucleus; cytosol; myelin sheath
Molecular Function: identical protein binding; protein binding; protein homodimerization activity; protease binding; protein phosphatase 2A binding; sequence-specific DNA binding; channel activity; protein heterodimerization activity; ubiquitin protein ligase binding; BH3 domain binding; channel inhibitor activity; transcription factor binding
Biological Process: focal adhesion formation; response to nicotine; positive regulation of catalytic activity; developmental growth; renal system process; pigment granule organization and biogenesis; protein polyubiquitination; response to toxin; response to glucocorticoid stimulus; T cell differentiation in the thymus; ear development; lymphoid progenitor cell differentiation; female pregnancy; positive regulation of multicellular organism growth; glomerulus development; negative regulation of mitochondrial depolarization; post-embryonic development; cochlear nucleus development; cellular response to glucose starvation; B cell receptor signaling pathway; negative regulation of myeloid cell apoptosis; regulation of mitochondrial membrane potential; negative regulation of ossification; positive regulation of B cell proliferation; regulation of transmembrane transporter activity; T cell homeostasis; negative regulation of neuron apoptosis; cell growth; defense response to virus; spleen development; response to drug; positive regulation of neuron maturation; release of cytochrome c from mitochondria; axon regeneration; regulation of protein homodimerization activity; actin filament organization; cell aging; digestive tract morphogenesis; regulation of calcium ion transport; positive regulation of cell growth; organ growth; induction of apoptosis via death domain receptors; DNA damage response, signal transduction resulting in induction of apoptosis; negative regulation of osteoblast proliferation; gland morphogenesis; regulation of mitochondrial membrane permeability; regulation of nitrogen utilization; metanephros development; oocyte development; negative regulation of apoptosis; B cell proliferation; negative regulation of autophagy; regulation of protein heterodimerization activity; behavioral fear response; melanin metabolic process; apoptosis; regulation of cell-matrix adhesion; negative regulation of retinal cell programmed cell death; regulation of protein stability; protein amino acid dephosphorylation; positive regulation of smooth muscle cell migration; response to radiation; B cell homeostasis; ovarian follicle development; positive regulation of skeletal muscle fiber development; positive regulation of melanocyte differentiation; melanocyte differentiation; response to gamma radiation; negative regulation of cell migration; regulation of viral genome replication; response to iron ion; transmembrane transport; negative regulation of cellular pH reduction; mesenchymal cell development; ossification; hair follicle morphogenesis; CD8-positive, alpha-beta T cell lineage commitment; thymus development; B cell lineage commitment; male gonad development; peptidyl-threonine phosphorylation; positive regulation of peptidyl-serine phosphorylation; humoral immune response; response to UV-B; endoplasmic reticulum calcium ion homeostasis; neuron apoptosis; peptidyl-serine phosphorylation; response to hydrogen peroxide; axonogenesis; ureteric bud branching; homeostasis of number of cells within a tissue; response to cytokine stimulus; innate immune response; negative regulation of cell growth; response to DNA damage stimulus; induction of apoptosis by oxidative stress
Research Articles on Bcl-2
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Product Notes
The Bcl-2 bcl2 (Catalog #AAA6277866) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Bcl-2, BH3 Domain Specific (BCL2, Apoptosis regulator Bcl-2) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Bcl-2 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), FLISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). IF: 1:10-50 FLISA: 1:1,000 IHC: 1:10-50 IP: 1:100-500 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Bcl-2 bcl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bcl-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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