Rabbit anti-Mouse Succinate Dehydrogenase Complex Subunit B Polyclonal Antibody | anti-SDHB antibody

Polyclonal Antibody to Succinate Dehydrogenase Complex Subunit B (SDHB)

Cat. Num. AAA2027081

• Gene Names: Sdhb; 0710008N11Rik
• Reactivity: Mouse
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation
• Purity: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Synonyms: Succinate Dehydrogenase Complex Subunit B; Polyclonal Antibody; Polyclonal Antibody to Succinate Dehydrogenase Complex Subunit B (SDHB); anti-SDHB antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Purity/Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
• Concentration: 0.79mg/ml (varies by lot)

• Applicable Applications for anti-SDHB antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, Immunoprecipitation (IP)
• Application Notes: Western blotting: 0.5-2 ug/mL;1:400-1600; Immunohistochemistry: 5-20 ug/mL;1:40-160; Immunocytochemistry: 5-20 ug/mL;1:40-160; ; Optimal working dilutions must be determined by end user.
• Organism Species: Mus musculus (Mouse)
• Source: Polyclonal antibody preparation
• Traits: Liquid
• Immunogen: Recombinant SDHB (Glu97~Ala282) expressed in E.coli
• Cross Reactivity: Mouse
• Preparation and Storage: Avoid repeated freeze/thaw cycles.; Store at 4ºC for frequent use.; Aliquot and store at -20ºC for 12 months.; ; Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Mouse Heart lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse SDHB Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded brain tissue))

NCBI and Uniprot Product Information

• NCBI GI #: 34328286
• NCBI GeneID: 67680
• NCBI Accession #: NP_075863.2
• NCBI GenBank Nucleotide #: NM_023374.4
• UniProt Accession #: Q9CQA3; Q3TF82; Q9DC91
• NCBI Official Full Name: succinate dehydrogenase
• NCBI Official Synonym Full Names: succinate dehydrogenase complex, subunit B, iron sulfur (Ip)
• NCBI Official Symbol: Sdhb
• NCBI Official Synonym Symbols: 0710008N11Rik
• NCBI Protein Information: succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial
• UniProt Protein Name: Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial
• Protein Family: Succinate dehydrogenase
• UniProt Gene Name: Sdhb
• UniProt Synonym Gene Names: Ip

Uniprot Description

SDHB: Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Defects in SDHB are a cause of susceptibility to pheochromocytoma (PCC). A catecholamine-producing tumor of chromaffin tissue of the adrenal medulla or sympathetic paraganglia. The cardinal symptom, reflecting the increased secretion of epinephrine and norepinephrine, is hypertension, which may be persistent or intermittent. Defects in SDHB are the cause of paragangliomas type 4 (PGL4). A neural crest tumor usually derived from the chromoreceptor tissue of a paraganglion. Paragangliomas are most commonly located in the head and neck region, specifically at the carotid bifurcation, the jugular foramen, the vagal nerve, and in the middle ear. Defects in SDHB are a cause of paraganglioma and gastric stromal sarcoma (PGGSS); also called Carney-Stratakis syndrome. Gastrointestinal stromal tumors may be sporadic or inherited in an autosomal dominant manner, alone or as a component of a syndrome associated with other tumors, such as in the context of neurofibromatosis type 1 (NF1). Patients have both gastrointestinal stromal tumors and paragangliomas. Susceptibility to the tumors was inherited in an apparently autosomal dominant manner, with incomplete penetrance. Defects in SDHB are a cause of Cowden-like syndrome (CWDLS). Cowden-like syndrome is a cancer predisposition syndrome associated with elevated risk for tumors of the breast, thyroid, kidney and uterus. Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.

Protein type: Carbohydrate Metabolism - citrate (TCA) cycle; EC 1.3.5.1; Energy Metabolism - oxidative phosphorylation; Mitochondrial; Oxidoreductase

Chromosomal Location of Human Ortholog: 4|4 D3

Cellular Component: membrane; mitochondrial inner membrane; mitochondrial respiratory chain complex II; mitochondrion; nucleoplasm; plasma membrane

Molecular Function: 2 iron, 2 sulfur cluster binding; 3 iron, 4 sulfur cluster binding; 4 iron, 4 sulfur cluster binding; electron transfer activity; iron-sulfur cluster binding; metal ion binding; oxidoreductase activity; succinate dehydrogenase (ubiquinone) activity

Biological Process: aerobic respiration; succinate metabolic process; transport; tricarboxylic acid cycle