Western Blot (WB)
(Western Blot: Sample: Recombinant F1+2, Mouse.)
Rabbit anti-Mouse Prothrombin Fragment 1+2 Antibody | anti-F1+2 antibody
Polyclonal Antibody to Prothrombin Fragment 1+2 (F1+2)
Immunohistochemistry: 5-20 ug/mL
Immunocytochemistry: 5-20 ug/mL
Optimal working dilutions must be determined by end user.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 24 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition
Western Blot (WB)
(Western Blot: Sample: Recombinant F1+2, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant F1+2, Mouse.)
Western Blot
(Western Blot Sample: Mouse SerumPrimary Ab: 5ug/ml Rabbit Anti-Mouse F1 2 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Western Blot
(Western Blot Sample: Mouse SerumPrimary Ab: 5ug/ml Rabbit Anti-Mouse F1 2 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Western Blot
(Western Blot; Sample: Mouse Blood Cells lysatePrimary Ab: 5ug/ml Rabbit Anti-Mouse F1 2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Western Blot
(Western Blot; Sample: Mouse Blood Cells lysatePrimary Ab: 5ug/ml Rabbit Anti-Mouse F1 2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a vitamin K-dependent glycoprotein coagulation factor that plays an important role in the process of blood coagulation and hemostasis. The encoded protein is an inactive zymogen that undergoes enzymatic cleavage by the coagulation factor Xa to form an active serine protease that converts soluble fibrinogen to insoluble fibrin clot. Most of the mice lacking the encoded protein die at an embryonic stage due to defects in yolk sac vasculature, while the rare nenonates succumb to hemorrhage on the first postnatal day. [provided by RefSeq, Apr 2015]
Uniprot Description
prothrombin: Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Defects in F2 are the cause of factor II deficiency (FA2D). It is a very rare blood coagulation disorder characterized by mucocutaneous bleeding symptoms. The severity of the bleeding manifestations correlates with blood factor II levels. Genetic variations in F2 may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Defects in F2 are the cause of thrombophilia due to thrombin defect (THPH1). It is a multifactorial disorder of hemostasis characterized by abnormal platelet aggregation in response to various agents and recurrent thrombi formation. A common genetic variation in the 3-prime untranslated region of the prothrombin gene is associated with elevated plasma prothrombin levels and an increased risk of venous thrombosis. Defects in F2 are associated with susceptibility to pregnancy loss, recurrent, type 2 (RPRGL2). A common complication of pregnancy, resulting in spontaneous abortion before the fetus has reached viability. The term includes all miscarriages from the time of conception until 24 weeks of gestation. Recurrent pregnancy loss is defined as 3 or more consecutive spontaneous abortions. Belongs to the peptidase S1 family.
Protein type: Apoptosis; EC 3.4.21.5; Protease; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 2 E1|2 50.63 cM
Cellular Component: blood microparticle; external side of plasma membrane; extracellular exosome; extracellular matrix; extracellular region; extracellular space
Molecular Function: calcium ion binding; heparin binding; hydrolase activity; lipopolysaccharide binding; peptidase activity; protein binding; receptor binding; serine-type endopeptidase activity; serine-type peptidase activity
Biological Process: acute-phase response; antimicrobial humoral immune response mediated by antimicrobial peptide; blood coagulation; cell surface receptor signaling pathway; cytolysis by host of symbiont cells; fibrinolysis; hemostasis; negative regulation of astrocyte differentiation; negative regulation of cytokine production involved in inflammatory response; negative regulation of proteolysis; neutrophil mediated killing of gram-negative bacterium; platelet activation; positive regulation of blood coagulation; positive regulation of cell growth; positive regulation of cell proliferation; positive regulation of collagen biosynthetic process; positive regulation of lipid kinase activity; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of phospholipase C-activating G-protein coupled receptor signaling pathway; positive regulation of protein localization to nucleus; positive regulation of protein phosphorylation; positive regulation of reactive oxygen species metabolic process; positive regulation of release of sequestered calcium ion into cytosol; proteolysis; regulation of cell shape; regulation of cytosolic calcium ion concentration; regulation of gene expression; response to wounding
Research Articles on F1+2
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Product Notes
The F1+2 f2 (Catalog #AAA2027561) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Prothrombin Fragment 1+2 (F1+2) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Prothrombin Fragment 1+2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitaion (IP). Western blotting: 0.5-5 ug/mL Immunohistochemistry: 5-20 ug/mL Immunocytochemistry: 5-20 ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the F1+2 f2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Prothrombin Fragment 1+2, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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