Goat STEM CELL FACTOR Polyclonal Antibody | anti-KITLG antibody

GOAT ANTI HUMAN STEM CELL FACTOR

Cat. Num. AAA221498

• Gene Names: KITLG; SF; MGF; SCF; FPH2; KL-1; Kitl; SHEP7
• Applications: ELISA, Functional Assay, Immunohistochemistry, Western Blot
• Synonyms: STEM CELL FACTOR; Polyclonal Antibody; GOAT ANTI HUMAN STEM CELL FACTOR; anti-KITLG antibody

• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - lyophilised
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 273

• Applicable Applications for anti-KITLG antibody: ELISA (EIA), Functional Assays (FN), Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: ELISA: Minimum Dilution: 0.5; Maximum Dilution: 2.0ug/ml;; Functional Assays: Minimum Dilution: 0.008; Maximum Dilution: 0.012ug/ml;; Immunohistology - Paraffin: Application Note: This product requires enzyme mediated antigen retrieval prior to staining of parrafin sections. Proteinase K is recomended for this purpose.; Successful heat mediated antigen retrieval has also been accomplished using Tris/EDTA buffer (pH9.0).; ; Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2ug/ml
• Reconstitution: Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
• Immunogen: Recombinant human stem cell factor.; Buffer Solution
• Antiserum Preparation: Antisera to human stem cell factor were raised by repeated immunisations of goats with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution.

Testing Data #1

(Formalin fixed, paraffin embedded human pancreatic adenocarcinoma stained with Rabbit anti Human SCF (MBS221498) following enzyme mediated antigen retrieval using proteinase K)

Testing Data #2

(Formalin fixed, paraffin embedded human pancreatic adenocarcinoma stained with Rabbit anti Human SCF (MBS221498) following enzyme mediated antigen retrieval using proteinase K)

Testing Data #3

(Formalin fixed, paraffin embedded human pancreatic adenocarcinoma stained with Rabbit anti Human SCF (MBS221498) following heat mediated antigen retrieval using EDTA buffer (pH9.0))

Testing Data #4

(Formalin fixed, paraffin embedded human pancreatic adenocarcinoma stained with Rabbit anti Human SCF (MBS221498) following enzyme mediated antigen retrieval using proteinase K)

Related Product Information for anti-KITLG antibody

Goat anti Human Stem Cell Factor antibody recognizes human stem cell factor (SCF), a type I transmembrane protein and haematopoietic growth factor, which acts in synergy with other growth factors and signals through the c-Kit receptor. Interaction between SCF and the receptor tyrosine kinase c-Kit is an essential step for the proliferation, survival and differentiation of haematopoietic progenitor cells, mast cells, myelanocytes and germ cells, and plays an important role in follicular development. The association between SCF and c-Kit is also involved with the progression and invasiveness of tumours, and deficiencies of either SCF or c-Kit result in mast cell deficiency and macrocytic anaemia.

NCBI and Uniprot Product Information

• NCBI GI #: 4505175
• NCBI GeneID: 4254
• NCBI Accession #: NP_000890.1
• NCBI GenBank Nucleotide #: NM_000899.4
• UniProt Accession #: P21583; Q16487; Q68DZ2; Q7M4N8; Q9UQK7; A0AV09; A8K2Q4; B7ZLM4
• Molecular Weight: 26,667 Da
• NCBI Official Full Name: kit ligand isoform b
• NCBI Official Synonym Full Names: KIT ligand
• NCBI Official Symbol: KITLG
• NCBI Official Synonym Symbols: SF; MGF; SCF; FPH2; KL-1; Kitl; SHEP7
• NCBI Protein Information: kit ligand; c-Kit ligand; familial progressive hyperpigmentation 2; mast cell growth factor; steel factor; stem cell factor
• UniProt Protein Name: Kit ligand
• Protein Family: Kit ligand
• UniProt Gene Name: KITLG
• UniProt Synonym Gene Names: MGF; SCF; MGF; SCF; sKITLG
• UniProt Entry Name: SCF_HUMAN

NCBI Description

This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

SCF: Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. Homodimer, non-covalently linked (Probable). Heterotetramer with KIT, binding two KIT molecules; thereby mediates KIT dimerization and subsequent activation by autophosphorylation. Belongs to the SCF family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q22

Cellular Component: extracellular space; cytoskeleton; cytoplasm; extracellular region; plasma membrane; integral to membrane

Molecular Function: protein binding; growth factor activity; cytokine activity; stem cell factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; embryonic hemopoiesis; male gonad development; germ cell programmed cell death; signal transduction; positive regulation of MAP kinase activity; cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of melanocyte differentiation; negative regulation of mast cell apoptosis; innate immune response; positive regulation of myeloid leukocyte differentiation; positive regulation of Ras protein signal transduction; cell adhesion; neural crest cell migration; positive regulation of DNA replication

Disease: Hyperpigmentation, Familial Progressive, 2; Skin/hair/eye Pigmentation, Variation In, 7