Western Blot (WB)
(Anti-p53R2 antibody, MBS176302, Western blottingLane 1: Rat Thymus Tissue LysateLane 2: MCF-7 Cell LysateLane 3: A431 Cell LysateLane 4: HELA Cell Lysate )
Rabbit p53R2 Polyclonal Antibody | anti-RRM2B antibody
Anti-p53R2 antibody
Western Blot (WB)
(Anti-p53R2 antibody, MBS176302, Western blottingLane 1: Rat Thymus Tissue LysateLane 2: MCF-7 Cell LysateLane 3: A431 Cell LysateLane 4: HELA Cell Lysate )
Western Blot (WB)
(Anti-p53R2 antibody, MBS176302, Western blottingLane 1: Rat Thymus Tissue LysateLane 2: MCF-7 Cell LysateLane 3: A431 Cell LysateLane 4: HELA Cell Lysate )
Background: RRM2B(Ribonucleotide Reductase, M2 B), also known as P53R2, is an enzyme that in humans is encoded by the RRM2B gene. Tanaka et al.(2000) mapped the p53R2 gene to chromosome 8q23.1 by fluorescence in situ hybridization. Tanaka et al.(2000) found that expression of p53R2, but not R2, was induced by ultraviolet and gamma-irradiation and adriamycin treatment in a wildtype p53-dependent manner. Induction of p53R2 in p53-deficient cells caused G2/M arrest and protected cells from death in response to adriamycin. Inhibition of endogenous p53R2 expression in cells that had an intact p53-dependent DNA damage checkpoint reduced ribonucleotide reductase activity, DNA repair, and cell survival after exposure to various genotoxins.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the small subunit of a p53-inducible ribonucleotide reductase. This heterotetrameric enzyme catalyzes the conversion of ribonucleoside diphosphates to deoxyribonucleoside diphosphates. The product of this reaction is necessary for DNA synthesis. Mutations in this gene have been associated with autosomal recessive mitochondrial DNA depletion syndrome, autosomal dominant progressive external ophthalmoplegia-5, and mitochondrial neurogastrointestinal encephalopathy. Alternatively spliced transcript variants have been described.[provided by RefSeq, Feb 2010]
Uniprot Description
RRM2B: Plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. Supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. Contains an iron-tyrosyl free radical center required for catalysis. Forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage. Defects in RRM2B are the cause of mitochondrial DNA depletion syndrome type 8A (MTDPS8A). A disorder due to mitochondrial dysfunction characterized by various combinations of neonatal hypotonia, neurological deterioration, respiratory distress, lactic acidosis, and renal tubulopathy. Defects in RRM2B are the cause of mitochondrial DNA depletion syndrome type 8B (MTDPS8B). A disease due to mitochondrial dysfunction and characterized by ophthalmoplegia, ptosis, gastrointestinal dysmotility, cachexia, peripheral neuropathy. Defects in RRM2B are the cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 5 (PEOA5). A disorder characterized by progressive weakness of ocular muscles and levator muscle of the upper eyelid. In a minority of cases, it is associated with skeletal myopathy, which predominantly involves axial or proximal muscles and which causes abnormal fatigability and even permanent muscle weakness. Ragged-red fibers and atrophy are found on muscle biopsy. A large proportion of chronic ophthalmoplegias are associated with other symptoms, leading to a multisystemic pattern of this disease. Additional symptoms are variable, and may include cataracts, hearing loss, sensory axonal neuropathy, ataxia, depression, hypogonadism, and parkinsonism. Belongs to the ribonucleoside diphosphate reductase small chain family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Oxidoreductase; Other Amino Acids Metabolism - glutathione; EC 1.17.4.1
Chromosomal Location of Human Ortholog: 8q23.1
Cellular Component: nucleoplasm; cytoplasm
Molecular Function: metal ion binding; ribonucleoside-diphosphate reductase activity
Biological Process: deoxyribonucleoside diphosphate metabolic process; renal system process; nucleobase, nucleoside and nucleotide interconversion; nucleobase, nucleoside and nucleotide metabolic process; deoxyribonucleotide biosynthetic process; mitochondrial DNA replication; response to oxidative stress; deoxyribonucleoside triphosphate metabolic process; kidney development; DNA repair
Disease: Mitochondrial Dna Depletion Syndrome 8a (encephalomyopathic Type With Renal Tubulopathy); Progressive External Ophthalmoplegia With Mitochondrial Dna Deletions, Autosomal Dominant, 5
Research Articles on RRM2B
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Product Notes
The RRM2B rrm2b (Catalog #AAA176302) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-p53R2 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's p53R2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the RRM2B rrm2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p53R2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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