SCF active protein

Human SCF

Cat. Num. AAA692177

• Gene Names: KITLG; SF; MGF; SCF; FPH2; KL-1; Kitl; SHEP7
• Reactivity: Human
• Purity: > 98% by SDS-PAGE & silver stain
• Synonyms: SCF; Human SCF; Recombinant Human Stem Cell Factor (SCF); Mast cell growth factor; Stem cell factor; c-Kit ligand; SCF active protein

• Host: Insect Cells
• Reactivity: Human
• Purity/Purification: > 98% by SDS-PAGE & silver stain
• Form/Format: Lyophilized
• Sequence: N-Terminal Sequence: UNDER WORK!
• Sequence Length: 165

• Buffer: PBS
• Preparation and Storage: The lyophilized human SCF, though stable at room temperature, is best stored desiccated below 0 degree C. Reconstituted human SCF should be stored in working aliquots at -20 degree C. Avoid repeated freeze-thaw cycles.

Testing Data

Testing Data #2

Related Product Information for SCF active protein

Stem Cell Factor (SCF), a 18,4 kDa protein consisting of 164 amino acid residues (Asp26-Ala189) and fused to a C-terminal His-tag (6x His), is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors. This pleiotropic cytokine, alternately known as mast cell growth factor (MGF) and steel-factor (SLF), plays essential roles in gametogenesis, melanogenesis and early stages of hematopoiesis. In vitro and in vivo, SCF can stimulate the proliferation of mature, as well as the proliferation and maturation of immature, mast cells. On purified primitive human and mouse hematopoietic precursors, SCF acts in a synergistic manner with various growth factors, such as IL-1, IL-3, IL-6, IL-7, and Epo, to induce myeloid, erythroid and lymphoid lineage colony formation. The finding that SCF is also expressed in the nervous system suggests a possible role for SCF in the development of the nervous system. The cDNA sequences for human, mouse and rat SCF encode transmembrane proteins which are composed of a signal peptide, a 189 amino acid extracellular domain, a hydrophobic transmembrane domain and an intracellular domain. Native SCF can exist either as the membrane bound form or as a soluble form consisting of the first 164 or 165 amino acids of the extracellular domain. The soluble form is believed to be a proteolytic cleavage product of the transmembrane protein. Both the soluble and the transmembrane form of SCF have growth factor activities. Native soluble SCF is a heavily N- and O-glycosylated protein which exists as a non-covalently associated dimer in solution. All four cysteine residues of SCF monomers are involved in intramolecular disulfide bonds. Murine or rat soluble SCF is highly homologous to human soluble SCF (approximately 80%). Whereas both rat and mouse SCF are active on human cells, the human protein is much less active on mouse or rat cells.

Product Categories/Family for SCF active protein

Cytokines & Growth Factors

References

1. Ashman LK Int J Biochem Cell Biol 31 (1999); 2. Sette et al, Int J Dev Biol 44 (2000); 3. Yoshida et al, J Invest Dermatol Symp Proc 6 (2001); 4. Erlandsson et al, Exp Cell Res 301 (2004); 5. Kapur et al, Blood 100 (2002); 6. Wang et al, Arterioscler Thromb Vasc Biol 27 (2007); 7. Bashamboo et al, J Cell Sci 119 (2006); 8. Reber et al, Eur J Pharmacol 533 (2006); 9. Huang et al, Cell 63 (1990); 10. Arakawa et al, J Biol Chem 266 (1991); 11. Majumdar et al, J Biol Chem 269 (1994); 12. Brannan et al, Proc Natl Acad Sci (1991); 13. Flanagan et al, Cell 64 (1991); 14. Martin et al, Cell 63 (1990); 15. Lemmon et al, J Biol Chem 272 (1997); 16. Kanellakis et al, Cardiovasc Res 70 (2006)

NCBI and Uniprot Product Information

• NCBI GI #: 4505175
• NCBI GeneID: 4254
• NCBI Accession #: NP_000890.1
• NCBI GenBank Nucleotide #: NM_000899.4
• UniProt Accession #: P21586; Q16487; Q68DZ2; Q7M4N8; Q9UQK7; A0AV09; A8K2Q4; B7ZLM4; P21583
• Molecular Weight: 20.0-23.0 kDa
• NCBI Official Full Name: kit ligand isoform b
• NCBI Official Synonym Full Names: KIT ligand
• NCBI Official Symbol: KITLG
• NCBI Official Synonym Symbols: SF; MGF; SCF; FPH2; KL-1; Kitl; SHEP7
• NCBI Protein Information: kit ligand; c-Kit ligand; steel factor; stem cell factor; mast cell growth factor; familial progressive hyperpigmentation 2
• UniProt Protein Name: Kit ligand
• Protein Family: SCF-associated factor
• UniProt Gene Name: KITLG
• UniProt Synonym Gene Names: MGF; SCF; MGF; SCF; sKITLG
• UniProt Entry Name: SCF_HUMAN

NCBI Description

This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.

Subunit structure: Homodimer, non-covalently linked

Probable. Heterotetramer with KIT, binding two KIT molecules; thereby mediates KIT dimerization and subsequent activation by autophosphorylation.

Subcellular location: Isoform 1: Cell membrane; Single-pass type I membrane protein Ref.9. Isoform 2: Cell membrane; Single-pass type I membrane protein

By similarity. Cytoplasm › cytoskeleton

By similarity Ref.9. Soluble KIT ligand: Secreted Ref.9.

Developmental stage: Acts in the early stages of hematopoiesis.

Post-translational modification: A soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain.Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97. Ref.9A soluble form exists as a cleavage product of the extracellular domain.

Polymorphism: Genetic variants in KITLG define the skin/hair/eye pigmentation variation locus 7 (SHEP7) [

MIM:611664]. Hair, eye and skin pigmentation are among the most visible examples of human phenotypic variation, with a broad normal range that is subject to substantial geographic stratification. In the case of skin, individuals tend to have lighter pigmentation with increasing distance from the equator. By contrast, the majority of variation in human eye and hair color is found among individuals of European ancestry, with most other human populations fixed for brown eyes and black hair.

Involvement in disease: Familial progressive hyperpigmentation (FPH) [MIM:145250]: A disorder characterized by hyperpigmented patches in the skin, present in early infancy and increasing in size and number with age.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.18

Sequence similarities: Belongs to the SCF family.