Rabbit anti-Human STEAP1 Polyclonal Antibody | anti-STEAP1 antibody

STEAP1, NT (STEAP1, PRSS24, STEAP, Metalloreductase STEAP1, Six-transmembrane epithelial antigen of prostate 1) (MaxLight 490)

Cat. Num. AAA6351934

• Gene Names: STEAP1; STEAP; PRSS24
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: STEAP1; Polyclonal Antibody; NT (STEAP1; PRSS24; STEAP; Metalloreductase STEAP1; Six-transmembrane epithelial antigen of prostate 1) (MaxLight 490); Six-transmembrane epithelial antigen of prostate 1; anti-STEAP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human STEAP1.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-STEAP1 antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 2-31 in the N-terminal region of human STEAP1.
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in WiDr cell line lysates (35ug/lane) usingMBS6009010. This demonstrates that MBS6009010 detected the STEAP1 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle using MBS6009010 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of 042391for immunohistochemistry.)

Product Categories/Family for anti-STEAP1 antibody

Antibodies; Transmembrane Proteins

References

1. Valenti, M.T., et al. J. Endocrinol. Invest. 33(4):244-249(2010). 2. Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010). 3. Vaghjiani, R.J., et al. Tissue Eng Part A 15(8):2073-2083(2009). 4. Mavaddat, N., et al. Cancer Epidemiol. Biomarkers Prev. 18(1):255-259(2009). 5. Maia, C.J., et al. Endocrine 34 (1-3), 108-116 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 9558759
• NCBI GeneID: 26872
• NCBI Accession #: NP_036581.1
• NCBI GenBank Nucleotide #: NM_012449.2
• UniProt Accession #: Q9UHE8; O95034; A4D1E0
• Molecular Weight: 39,851 Da
• NCBI Official Full Name: metalloreductase STEAP1
• NCBI Official Synonym Full Names: six transmembrane epithelial antigen of the prostate 1
• NCBI Official Symbol: STEAP1
• NCBI Official Synonym Symbols: STEAP; PRSS24
• NCBI Protein Information: metalloreductase STEAP1; six-transmembrane epithelial antigen of prostate 1
• UniProt Protein Name: Metalloreductase STEAP1
• Protein Family: Metalloreductase
• UniProt Gene Name: STEAP1
• UniProt Synonym Gene Names: PRSS24; STEAP
• UniProt Entry Name: STEA1_HUMAN

NCBI Description

This gene is predominantly expressed in prostate tissue, and is found to be upregulated in multiple cancer cell lines. The gene product is predicted to be a six-transmembrane protein, and was shown to be a cell surface antigen significantly expressed at cell-cell junctions. [provided by RefSeq, Jul 2008]

Uniprot Description

STEAP1: Metalloreductase that has the ability to reduce both Fe(3+) to Fe(2+) and Cu(2+) to Cu(1+). Uses NAD(+) as acceptor. Belongs to the STEAP family.

Protein type: Cell adhesion; EC 1.16.1.-; Membrane protein, multi-pass; Membrane protein, integral; Oxidoreductase

Chromosomal Location of Human Ortholog: 7q21

Cellular Component: membrane; integral to plasma membrane; plasma membrane; endosome membrane; intercellular junction

Molecular Function: transporter activity; channel activity; metal ion binding; oxidoreductase activity

Biological Process: iron ion homeostasis; ion transport; transmembrane transport