Mouse anti-Human ATP6E/ATP6V1E1 Monoclonal Antibody | anti-ATP6E/ATP6V1E1 antibody

Anti-ATP6E/ATP6V1E1 Antibody (clone 4E11)

Cat. Num. AAA2401163

• Gene Names: ATP6V1E1; P31; Vma4; ATP6E; ARCL2C; ATP6E2; ATP6V1E
• Reactivity: Human
• Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified from ascites by Protein A
• Synonyms: ATP6E/ATP6V1E1; Monoclonal Antibody; Anti-ATP6E/ATP6V1E1 Antibody (clone 4E11); Rabbit Polyclonal (IgG) to Human ATP6E/ATP6V1E1; ATP6V1E1; ATP6E; ATP6E2; ATP6V1E; V-ATPase subunit E 1; V-ATPase; subunit E; V-ATPase 31 kDa subunit; p31; V-ATPase subunit E; VMA4; anti-ATP6E/ATP6V1E1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a, k
• Clone Number: 4E11
• Specificity: Human ATP6E/ATP6V1E1
• Purity/Purification: Purified from ascites by Protein A
• Form/Format: PBS, pH 7.4.
• Sequence Length: 226

• Applicable Applications for anti-ATP6E/ATP6V1E1 antibody: ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IF: 10 ug/ml; IHC-P: 10 ug/ml
• Immunogen: ATP6V1E1 (AAH04443, aa1-226 full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C or lower.; Aliquot to avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry (WB)

(Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to ATP6V1E1 on HeLa cell. [antibody concentration 10 ug/ml])

Western Blot (WB)

(Western Blot analysis of ATP6V1E1 expression in transfected 293T cell line by ATP6V1E1 monoclonal antibody (M02), clone 4E11.Lane 1: ATP6V1E1 transfected lysate(26.1 KDa).Lane 2: Non-transfected lysate.)

ELISA (EIA)

(Detection limit for recombinant GST tagged ATP6V1E1 is 1 ng/ml as a capture antibody.)

Related Product Information for anti-ATP6E/ATP6V1E1 antibody

ATP6E/ATP6V1E1 is a component of vacuolar ATPase (V-ATPase), amultisubunit enzyme that mediates acidification of eukaryoticintracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle protongradient generation. V-ATPase is composed of a cytosolic V1 domain and atransmembrane V0 domain.

Product Categories/Family for anti-ATP6E/ATP6V1E1 antibody

Transporter; ATPase - V type

NCBI and Uniprot Product Information

• NCBI GI #: 4502317
• NCBI GeneID: 529
• NCBI Accession #: NP_001687.1
• NCBI GenBank Nucleotide #: NM_001696.3
• UniProt Accession #: P36543; A8MUE4; A8MUN4
• Molecular Weight: 22,706 Da
• NCBI Official Full Name: V-type proton ATPase subunit E 1 isoform a
• NCBI Official Synonym Full Names: ATPase H+ transporting V1 subunit E1
• NCBI Official Symbol: ATP6V1E1
• NCBI Official Synonym Symbols: P31; Vma4; ATP6E; ARCL2C; ATP6E2; ATP6V1E
• NCBI Protein Information: V-type proton ATPase subunit E 1
• UniProt Protein Name: V-type proton ATPase subunit E 1
• UniProt Gene Name: ATP6V1E1
• UniProt Synonym Gene Names: ATP6E; ATP6E2; V-ATPase subunit E 1; p31

NCBI Description

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain E subunit isoforms. Pseudogenes for this gene have been found in the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.