Heparanase-1 Active Protein | HPSE active protein

Recombinant Human Heparanase-1 Active

Cat. Num. AAA147099

• Purity: > 95.0% on SDS-PAGE (Fig. 1, B); > 95% on RP-HPLC (Fig. 1, C)
• Synonyms: Heparanase-1; Recombinant Human Heparanase-1 Active; HPSE Active; HPSE active protein

• Purity/Purification: > 95.0% on SDS-PAGE (Fig. 1, B); > 95% on RP-HPLC (Fig. 1, C)
• Form/Format: 20 mM Acetate buffer; 750 mM NaCl; pH 5.4 per 1 µg of rhHPA1

• Source: Recombinant Human Heparanase 1, Active Protein (HepaOne®) is produced in CHO cells. The protein was purified by several orthogonal chromatography steps.
• Potency: The specific activity of HepaOne® in-house standard is about 0.7 Units (1 unit = 1 μmole of reducing ends of heparan sulfate substrate formed per minute per mg HepaOne® at 37°C). The enzymatic activity of each HepaOne® batch is comparable to the standard as determined by activity assay in which immobilized heparan, released due to heparanase activity, is quantified colorimetrically.; Recommended reaction buffer: 20 mM Citrate Phosphate buffer, pH 5.4; 50mM NaCl; 1mM CaCl2
• Patents: HepaOne® and its uses are protected by different patents and patent applications in the US and worldwide.
• Identity: HepaOne® is identified by Western blot analysis with polyclonal rabbit anti-HPA1 antibodies as 2 subunits of 8-kDa and 50-kDa (Fig. 1, A)
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Store at-80°C.

Testing Data

Related Product Information for HPSE active protein

Background: Heparanase Active Enzyme is produced in CHO cells. The protein is purified by several orthogonal chromatography steps.
Introduction: Heparanase is an endo beta-D-glucuronidase, which degrades heparan sulfate side chains of heparan sulfate proteoglycans (HSPGs) in the extracellular matrix. Heparanase plays an important role in ECM degradation, facilitating the migration and extravasation of tumor cells and inflammatory leukocytes (1, 2, 3). Upon degradation, heparanase releases growth factors and cytokines that stimulate cell proliferation and chemotaxis (4, 5). Heparanase is a heterodimer comprised of a 50 kDa subunit harboring the active site and a 8 kDa subunit. It is produced as a latent 65 kDa precursor and proteolytically processed to its active form (1, 6). Heparanase is highly expressed in myeloid leukocytes (i.e. neutrophils) in platelets and in human placenta. Human heparanase was found to be upregulated in various types of primary tumors, correlating in some cases with increased tumor invasiveness and vascularity and with poor prospective survival (7, 8).

Product Categories/Family for HPSE active protein

Enzymes; Heparanase; HPSE Active

References

1. Vlodavsky, I. et al.., Nat. Med. 5 (1999) 793-802.2. Vlodavsky, I and Friedman, Y., J. Clin. Invest. 108 (2001) 341-347.3. Parish, C.R. et al., Biochem. Biophys. Acta 1471 (2001) M99-M108.4. Vlodasvsky, I. et al., Cancer Metastasis Rev. 9, (1990) 203-226.5. Bashkin, P. et al., . Biochemistry 28 (1989), 1737-1743.6. Fairbanks, M.B. et al., J. Biol. Chem. 274 (1999) 29587- 29590.7. Koliopanos, A.et al., Cancer Res. 61 (1992) 4655-4659.8. Gohji, K. et al., Int. J. Cancer 95 (2001) 295-301