Rabbit anti-Human STAT1 phospho Ser727 Polyclonal Antibody | anti-STAT1 antibody

STAT1 phospho Ser727 antibody (pAb)

Cat. Num. AAA388519

• Gene Names: STAT1; CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91
• Reactivity: Human
• Applications: Dot Blot, Western Blot
• Synonyms: STAT1 phospho Ser727; Polyclonal Antibody; STAT1 phospho Ser727 antibody (pAb); CANDF7; ISGF-3; STAT91; anti-STAT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Form/Format: Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

• Applicable Applications for anti-STAT1 antibody: Dot Blot (DB), Western Blot (WB)
• Application Notes: Applications Validated:; WB: 1:500-1:1,000 dilution
• Conjugation: Phosphorylated
• Immunogen: This STAT1 phospho Ser727 antibody was raised against a synthetic peptide containing phospho-serine 727 of human STAT1.
• Abs Residue: Ser
• Preparation and Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul

Western Blot (WB)

(STAT1 phospho Ser727 pAb tested by Western blot. Lanes 1&4: Untreated HeLa whole-cell extract (20 ug). Lane 2&5: Whole-cell extract (20 ug) of HeLa cells treated with IFNa (2500U/ml for 30 min.). Lanes 3&6: Whole-cell extract (20 ug) of COS-7 cells treated with IFNg (1000U/ml for 15 min.). Lanes 1-3 were probed with at 1:500. Lanes 4-6 probed with (STAT1 alpha pAb) at 1:1000.)

Dot Blot (DB)

(STAT1 phospho Ser727 pAb tested by Dot blot. Dot blot analysis was used to confirm the specificity of 39633 for STAT1 phospho Ser727. Phosphorylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with 39633 at 1:500. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: unmodified Ser727 STAT1 peptide. Lane 2: phospho Ser727 STAT1 peptide. Lane 3: unmodified Tyr689 STAT2 peptide. Lane 4: phospho Tyr689 STAT2 peptide. Lane 5: unmodified Ser727 STAT3 peptide. Lane 6: phospho Ser727 STAT3 peptide. Lane 7: unmodified Tyr705 STAT3 peptide. Lane 8: phospho Tyr705 STAT3 peptide. Lane 9: unmodified Ser726 STAT5A/Ser731 STAT5B peptide. Lane 10: phospho Ser726 STAT5A/Ser731 STAT5B peptide. Lane 11: unmodified Tyr694 STAT5A/Tyr699 STAT5B peptide. Lane 12: phospho Tyr694 STAT5A/Tyr699 STAT5B)

Related Product Information for anti-STAT1 antibody

Short Description: Our STAT1 phospho Ser727 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Dot blot and Western blot; it has been shown to react with Human samples.

Background: STAT1 is a member of the STAT family of transcription factors. STATs undergo tyrosine and serine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. STAT1 is alternatively spliced to give rise to STAT1a and STAT1b, both of which mediate interferon a activity, but only STAT1a mediates interferon-g activity. STAT1 plays a critical role in various forms of cell death, both apoptotic and non-apoptotic. The mechanisms of action are both by transcriptional activation of target genes, and by direct association with apoptotic regulators, including TRADD, p53, and HDAC (HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6 and HDAC11). STAT1 target genes include the pro-inflammatory genes for iNOS, COX, VCAM, and ICAM, making STAT1 an attractive anti-inflammatory target.

Product Categories/Family for anti-STAT1 antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 6274552
• NCBI GeneID: 6772
• NCBI Accession #: NP_009330; AB_2793283
• NCBI GenBank Nucleotide #: NM_007315.3
• UniProt Accession #: P42224; Q53S88; Q53XW4; Q68D00; Q9UDL5; A8K989; B2RCA0; D2KFR8; D3DPI7
• Molecular Weight: 91 kDa
• NCBI Official Full Name: signal transducer and activator of transcription 1-alpha/beta isoform alpha
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 1, 91kDa
• NCBI Official Symbol: STAT1
• NCBI Official Synonym Symbols: CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91
• NCBI Protein Information: signal transducer and activator of transcription 1-alpha/beta; transcription factor ISGF-3 components p91/p84; signal transducer and activator of transcription-1; signal transducer and activator of transcription 1, 91kD
• UniProt Protein Name: Signal transducer and activator of transcription 1-alpha/beta
• Protein Family: Signal transducer and activator of transcription
• UniProt Gene Name: STAT1
• UniProt Entry Name: STAT1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

STAT1: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of certain receptor tyrosine kinases, GPCRs, and receptors for various interleukins and interferons. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 2q32.2

Cellular Component: nucleoplasm; axon; nuclear chromatin; cytoplasm; dendrite; nucleolus; cytosol; nucleus

Molecular Function: identical protein binding; signal transducer activity; protein binding; protein homodimerization activity; enzyme binding; double-stranded DNA binding; transcription factor activity; tumor necrosis factor receptor binding; CCR5 chemokine receptor binding

Biological Process: transcription from RNA polymerase II promoter; response to peptide hormone stimulus; response to cAMP; viral reproduction; blood circulation; apoptosis; positive regulation of transcription, DNA-dependent; positive regulation of smooth muscle cell proliferation; negative regulation of transcription from RNA polymerase II promoter; regulation of apoptosis; response to exogenous dsRNA; tumor necrosis factor-mediated signaling pathway; positive regulation of mesenchymal cell proliferation; negative regulation of viral protein levels in host cell; lipopolysaccharide-mediated signaling pathway; defense response to virus; response to nutrient; response to drug; cytokine and chemokine mediated signaling pathway; negative regulation of I-kappaB kinase/NF-kappaB cascade; JAK-STAT cascade; regulation of transcription from RNA polymerase II promoter; negative regulation of angiogenesis; response to hydrogen peroxide; cellular response to insulin stimulus; response to mechanical stimulus; response to cytokine stimulus; negative regulation of endothelial cell proliferation; endothelial cell migration; positive regulation of transcription from RNA polymerase II promoter

Disease: Immunodeficiency 31a; Immunodeficiency 31b; Immunodeficiency 31c