Western Blot (WB)
(STAT1a pAb tested by Western blot. Detection of STAT1a by Western blot analysis. STAT1a was detected in whole-cell extracts derived from T-47D cells using STAT1a rabbit polyclonal antibody at a 1:500 dilution. Several uncharacterized bands of approximately 78 and 140 kDa may also be revealed under the same conditions.)
Rabbit anti-Human STAT1a Polyclonal Antibody | anti-STAT1a antibody
STAT1a antibody (pAb)
WB: 1:500-1:1,000 dilution
For optimal results, primary antibody incubations should be performed at 37 degree C.
Western Blot (WB)
(STAT1a pAb tested by Western blot. Detection of STAT1a by Western blot analysis. STAT1a was detected in whole-cell extracts derived from T-47D cells using STAT1a rabbit polyclonal antibody at a 1:500 dilution. Several uncharacterized bands of approximately 78 and 140 kDa may also be revealed under the same conditions.)
Western Blot (WB)
(STAT1a pAb tested by Western blot. Detection of STAT1a by Western blot analysis. STAT1a was detected in whole-cell extracts derived from T-47D cells using STAT1a rabbit polyclonal antibody at a 1:500 dilution. Several uncharacterized bands of approximately 78 and 140 kDa may also be revealed under the same conditions.)
Background: STAT1 is a member of the STAT family of transcription factors. STATs undergo tyrosine and serine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. STAT1 is alternatively spliced to give rise to STAT1a and STAT1b, both of which mediate interferon a activity, but only STAT1a mediates interferon-g activity. STAT1 plays a critical role in various forms of cell death, both apoptotic and non-apoptotic. The mechanisms of action are both by transcriptional activation of target genes, and by direct association with apoptotic regulators, including TRADD, p53, and HDAC (HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6 and HDAC11). STAT1 target genes include the pro-inflammatory genes for iNOS, COX, VCAM, and ICAM, making STAT1 an attractive anti-inflammatory target.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
Uniprot Description
STAT1: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of certain receptor tyrosine kinases, GPCRs, and receptors for various interleukins and interferons. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 2q32.2
Cellular Component: nucleoplasm; axon; nuclear chromatin; cytoplasm; dendrite; nucleolus; cytosol; nucleus
Molecular Function: identical protein binding; signal transducer activity; protein binding; protein homodimerization activity; enzyme binding; double-stranded DNA binding; transcription factor activity; tumor necrosis factor receptor binding; CCR5 chemokine receptor binding
Biological Process: transcription from RNA polymerase II promoter; response to peptide hormone stimulus; response to cAMP; viral reproduction; blood circulation; apoptosis; positive regulation of transcription, DNA-dependent; positive regulation of smooth muscle cell proliferation; negative regulation of transcription from RNA polymerase II promoter; regulation of apoptosis; response to exogenous dsRNA; tumor necrosis factor-mediated signaling pathway; positive regulation of mesenchymal cell proliferation; negative regulation of viral protein levels in host cell; lipopolysaccharide-mediated signaling pathway; defense response to virus; response to nutrient; response to drug; cytokine and chemokine mediated signaling pathway; negative regulation of I-kappaB kinase/NF-kappaB cascade; JAK-STAT cascade; regulation of transcription from RNA polymerase II promoter; negative regulation of angiogenesis; response to hydrogen peroxide; cellular response to insulin stimulus; response to mechanical stimulus; response to cytokine stimulus; negative regulation of endothelial cell proliferation; endothelial cell migration; positive regulation of transcription from RNA polymerase II promoter
Disease: Immunodeficiency 31a; Immunodeficiency 31b; Immunodeficiency 31c
Research Articles on STAT1a
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Product Notes
The STAT1a stat1 (Catalog #AAA388345) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The STAT1a antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's STAT1a can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1:500-1:1,000 dilution For optimal results, primary antibody incubations should be performed at 37 degree C. Researchers should empirically determine the suitability of the STAT1a stat1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STAT1a, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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