Rabbit STAT1 alpha Polyclonal Antibody | anti-STAT1 alpha antibody

STAT1 alpha Antibody

Cat. Num. AAA9407110

• Gene Names: STAT1; CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunoprecipitation
• Purity: DEAE purified
• Synonyms: STAT1 alpha; Polyclonal Antibody; STAT1 alpha Antibody; STAT1a; anti-STAT1 alpha antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: No reaction to the 84 kDa STAT1a.
• Purity/Purification: DEAE purified
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 750

• Applicable Applications for anti-STAT1 alpha antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a peptide corresponding to amino acids near the carboxy terminus of human STAT1 alpha. The sequences differ from the murine corresponding sequences by four amino acids.
• Target Name: STAT1 alpha
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of STAT1 alpha in whole cell lysates from HeLa (H), Jurkat (J), A431 (A), K562 (K), and NIH3T3 (N) cells, with STAT1a antibody at 1 ug/mL.)

Immunocytochemistry (ICC)

(Immunocytochemistry of STAT1 alpha in HeLa cells with STAT1 alpha antibody at 10 ug/mL.)

Related Product Information for anti-STAT1 alpha antibody

STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5a, Stat5b, and Stat6), Stat1, Stat3, Stat5a, and Stat5b have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-a, IFN-b, IFN-g and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). STAT1 has two forms, the 91 kDa STAT1a and the 84 kDa STAT1b which are encoded by the same gene with splicing variant.

Product Categories/Family for anti-STAT1 alpha antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 6274552
• NCBI GeneID: 6772
• NCBI Accession #: NP_009330
• NCBI GenBank Nucleotide #: NM_007315.3
• Molecular Weight: 83,043 Da
• NCBI Official Full Name: signal transducer and activator of transcription 1-alpha/beta isoform alpha
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 1, 91kDa
• NCBI Official Symbol: STAT1
• NCBI Official Synonym Symbols: CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91
• NCBI Protein Information: signal transducer and activator of transcription 1-alpha/beta
• UniProt Protein Name: Signal transducer and activator of transcription 1-alpha/beta
• UniProt Gene Name: STAT1
• UniProt Entry Name: STAT1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

STAT1: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of certain receptor tyrosine kinases, GPCRs, and receptors for various interleukins and interferons. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 2q32.2

Cellular Component: nucleoplasm; axon; dendrite; cytoplasm; nuclear chromatin; nucleolus; cytosol; nucleus

Molecular Function: identical protein binding; signal transducer activity; protein binding; enzyme binding; protein homodimerization activity; double-stranded DNA binding; transcription factor activity; tumor necrosis factor receptor binding; CCR5 chemokine receptor binding

Biological Process: transcription from RNA polymerase II promoter; response to peptide hormone stimulus; response to cAMP; viral reproduction; blood circulation; apoptosis; positive regulation of smooth muscle cell proliferation; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; regulation of apoptosis; response to exogenous dsRNA; tumor necrosis factor-mediated signaling pathway; positive regulation of mesenchymal cell proliferation; negative regulation of viral protein levels in host cell; lipopolysaccharide-mediated signaling pathway; defense response to virus; response to nutrient; response to drug; cytokine and chemokine mediated signaling pathway; negative regulation of I-kappaB kinase/NF-kappaB cascade; JAK-STAT cascade; regulation of transcription from RNA polymerase II promoter; negative regulation of angiogenesis; response to hydrogen peroxide; cellular response to insulin stimulus; response to mechanical stimulus; response to cytokine stimulus; negative regulation of endothelial cell proliferation; endothelial cell migration; positive regulation of transcription from RNA polymerase II promoter

Disease: Immunodeficiency 31a; Immunodeficiency 31b; Immunodeficiency 31c