Rabbit anti-Human, Mouse STAT 2 Polyclonal Antibody | anti-Stat antibody

STAT 2, phosphorylated (Tyr689) (Signal Transducer and Activator of Transcription 2)

Cat. Num. AAA627744

• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: STAT 2; Polyclonal Antibody; phosphorylated (Tyr689) (Signal Transducer and Activator of Transcription 2); Anti -STAT 2; anti-Stat antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse STAT2 phosphorylated at Tyr689, Mr130kD.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide, 30% glycerol.

• Applicable Applications for anti-Stat antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:500-1:2000 detected phosphorylated STAT2 in HeLa whole cell RIPA lysate stimulated with interferon gamma (0.3ng/ml) for 16-20 hours and then treated with interferon alpha (2000 units/ml) for 20 minutes. HeLa whole cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with S7970-80 (1:200). Proteins were visualized using a goat anti-rabbit (HRP) secondary and a chemiluminescence detection system.
• Immunogen: Synthetic peptide corresponding to aa683-691, C-EEQRK[pY]LKH of mouse STAT2 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Chemiluminescence

(HeLa cell lysate and FN alpha/gamma treated HeLa cell lysate probed with MBS627744 (1:100). Proteins were visualized using an IgG (HRP) goat anti-rabbit secondary and a chemiluminescence detection system.)

Immunoprecipitation (IP)

(IFN alpha treated HeLa cell lysate immunoprecipitated with MBS627744.)

Testing Data

(HeLa cell lysate (Lane 1) and IFN alpha/gamma treated HeLa cell lysate (Lane 2) were probed with MBS627744 (1:100). Proteins were visualized using an IgG (HRP) donkey anti-rabbit secondary and a chemiluminescence detection system.)

Related Product Information for anti-Stat antibody

STAT (for signal transducers and activators of transcription) proteins can be selectively phosphorylated on tyrosine residues by Jak family kinases and some receptors with tyrosine kinase activity. The STAT proteins are highly conserved in their amino termini, but have divergent carboxy termini, which are thought to be essential for their selective activity. Seven STAT proteins have been described (STAT1a, STAT1b, STAT2, STAT3, STAT4, STAT5, and STAT6) and range in molecular weight from 84-113kD. Interferon-a promotes phosphorylation of STAT1 and STAT2, which then form heterodimers that interact with the 48kD DNA binding protein ISGF3. This complex moves to the nucleus where it binds to interferon-stimulated response elements and activates transcription of specific genes. In contrast to interferon-a signaling, interferon-g only induces phosphorylation of STAT1a or b. STAT proteins may be involved in the intracellular signaling of many cytokines and growth factors via the activation of gene expression.

Product Categories/Family for anti-Stat antibody

Antibodies; Abs to Transcription Factors; STAT

NCBI and Uniprot Product Information

• NCBI GI #: 3860293
• UniProt Accession #: Q7QDU4; O97164
• Molecular Weight: 83,879 Da
• NCBI Official Full Name: stat
• UniProt Protein Name: Signal transducer and transcription activator
• Protein Family: Signal transducer and transcription activator
• UniProt Gene Name: Stat
• UniProt Entry Name: STAT_ANOGA

Uniprot Description

Function: Signal transduction and activation of transcription. Plays an important role in the segmental pattern formation in the early embryo by activating specific stripes of pair rule gene expression

By similarity. The STAT pathway directly participates in immune responses in insects. Ref.1

Subunit structure: Forms a homodimer or a heterodimer with a related family member

By similarity.

Subcellular location: Cytoplasm

By similarity. Nucleus

By similarity. Note: Translocated into the nucleus in response to phosphorylation

By similarity.

Tissue specificity: Expressed in hemocytes, pericardial cells, midgut, skeletal muscle and fat body cells. Ref.1

Developmental stage: Expressed at all developmental stages. Ref.1

Induction: Bacterial challenge results in nuclear translocation in fat body cells and induction of DNA-binding activity that recognizes a STAT target site. In vitro treatment with pervanadate (vanadate and H2O2) translocates protein to the nucleus in midgut epithelial cells. Ref.1

Sequence similarities: Belongs to the transcription factor STAT family.Contains 1 SH2 domain.