Western Blot (WB)
(WB: Untreated (Lane 1) or CNTF-treated (Lane 2) SK-N-MC cell lysate was probed with MBS630449 (1:1000).)
Rabbit anti-Mouse STAT 1 Polyclonal Antibody
Rabbit Anti-STAT 1, phosphorylated (Tyr701) (Signal Transducer and Activator of Transcription 1)
Reactivity
Mouse
Applications
Western Blot
Purity
Purified by immunoaffinity chromatography.
Synonyms
STAT 1; Polyclonal Antibody; Rabbit Anti-STAT 1; phosphorylated (Tyr701) (Signal Transducer and Activator of Transcription 1); Anti -STAT 1; anti-STAT 1 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse phosphorylated STAT1 at ~92kD. Species Crossreactivity: human, rat and chicken.
Purity/Purification
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, 5mg/ml BSA, 0.09% sodium azide, 50% glycerol.
Applicable Applications for anti-STAT 1 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 1:500-1:200 dilution detects phosphorylated STAT1 in RIPA lysates from CNTF-treated SK-N-MC cells. 20ug of non-treated or CNTF-treated SK-N-MC cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with S7969-08. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Dilution: Western Blot: 1:500-1:200 dilution detects phosphorylated STAT1 in RIPA lysates from CNTF-treated SK-N-MC cells. 20ug of non-treated or CNTF-treated SK-N-MC cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with S7969-08. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunogen
Synthetic peptide corresponding to aa694-705, C-DDPKRTG[pY]IKTE of mouse STAT1, containing a N-terminal cysteine conjugated to KLH.
Control
Mouse 3T3-L1 adipocytes stimulated with leukemia inhibitory factor (LIF)
Cross-Reactivity
Human, Rat, and Chicken
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(WB: Untreated (Lane 1) or CNTF-treated (Lane 2) SK-N-MC cell lysate was probed with MBS630449 (1:1000).)
Western Blot (WB)
(WB: Untreated (Lane 1) or CNTF-treated (Lane 2) SK-N-MC cell lysate was probed with MBS630449 (1:1000).)
Related Product Information for anti-STAT 1 antibody
STAT proteins (Signal Transduction and Activators of Transcription) are latent cytoplasmic transcription factors that have the dual function of signal transduction and activation of transcription. STATs are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus. The N-terminal region is highly homologous among the STAT proteins and surrounds a completely conserved arginine residue. STATs are a part of the JAK-STAT signaling pathway - a major pathway of the immune system. All cytokines transduce critical signals through this pathway. STAT 1, is activated by a number of different ligands, including IFNalpha, IFNgamma, EGF, PDGF and IL-6. Phosphorylation of tyrosine 701 is required for STAT 1 dissociation from IFNGR1, homodimerization, and nuclear translocation. Tyrosine 701 phosphorylation impairment results in loss of STAT 1 functions.
Product Categories/Family for anti-STAT 1 antibody
NCBI and Uniprot Product Information
UniProt Accession #
Similar Products
Product Notes
The STAT 1 (Catalog #AAA630449) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Anti-STAT 1, phosphorylated (Tyr701) (Signal Transducer and Activator of Transcription 1) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's STAT 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1:500-1:200 dilution detects phosphorylated STAT1 in RIPA lysates from CNTF-treated SK-N-MC cells. 20ug of non-treated or CNTF-treated SK-N-MC cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with S7969-08. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Researchers should empirically determine the suitability of the STAT 1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STAT 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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