Rabbit anti-Human STAT 2, phosphorlated Polyclonal Antibody | anti-Stat antibody

STAT 2, phosphorlated (Tyr689) (Signal Transducer and Activator of Transcription 2) (Carboxyfluorescein)

Cat. Num. AAA615901

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: STAT 2; phosphorlated; Polyclonal Antibody; phosphorlated (Tyr689) (Signal Transducer and Activator of Transcription 2) (Carboxyfluorescein); Anti -STAT 2; anti-Stat antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human STAT 2 phosphoryated at Tyr689.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.1% sodium. Labeled with Carboxyfluorescein.

• Applicable Applications for anti-Stat antibody: Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Flow Cytometry.; Dilution: Flow Cytometry: Use 10ul to label 5x10e5 cells.; Excitation: 488nm; Emission: 515-545nm
• Immunogen: Phosphopeptide containing Y689 site of human STAT2
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Protect from light.

Flow Cytometry (FC/FACS)

(Intracellular Staining by Flow Cytometry-Detection of Phospho-STAT2 (Y689) in Daudi Human Cell Line by Flow Cytometry.)

Related Product Information for anti-Stat antibody

Signal Transducer and Activator of Transcription 2 (STAT2) is a 98kD member of the STAT family of cytoplasmic transcription factors. STAT members generally mediate cytokine, growth factor and hormone receptor signal transduction. STAT2 is associated with type I (a and b--) interferon signaling. All STATs contain an N-terminal oligomerization domain, a DNA-binding domain, and an SH2-association region. STAT2 is phosphorylated at Y689 by receptor-associated Janus kinases (JAKs) leading to STAT2 dimerization and subsequent translocation to the nucleus to activate gene transcription.

Product Categories/Family for anti-Stat antibody

Antibodies; Abs to Transcription Factors; STAT

NCBI and Uniprot Product Information

• NCBI GI #: 3860293
• UniProt Accession #: Q7QDU4; O97164
• Molecular Weight: 83,879 Da
• NCBI Official Full Name: stat
• UniProt Protein Name: Signal transducer and transcription activator
• Protein Family: Signal transducer and transcription activator
• UniProt Gene Name: Stat
• UniProt Entry Name: STAT_ANOGA

Uniprot Description

Function: Signal transduction and activation of transcription. Plays an important role in the segmental pattern formation in the early embryo by activating specific stripes of pair rule gene expression

By similarity. The STAT pathway directly participates in immune responses in insects. Ref.1

Subunit structure: Forms a homodimer or a heterodimer with a related family member

By similarity.

Subcellular location: Cytoplasm

By similarity. Nucleus

By similarity. Note: Translocated into the nucleus in response to phosphorylation

By similarity.

Tissue specificity: Expressed in hemocytes, pericardial cells, midgut, skeletal muscle and fat body cells. Ref.1

Developmental stage: Expressed at all developmental stages. Ref.1

Induction: Bacterial challenge results in nuclear translocation in fat body cells and induction of DNA-binding activity that recognizes a STAT target site. In vitro treatment with pervanadate (vanadate and H2O2) translocates protein to the nucleus in midgut epithelial cells. Ref.1

Sequence similarities: Belongs to the transcription factor STAT family.Contains 1 SH2 domain.