Rabbit STAT 1 Polyclonal Antibody | anti-SOAT1 antibody

STAT 1, phosphorylated (Ser727) (Signal Transducer and Activator of Transcription 1-alpha/beta, Transcription Factor ISGF-3 Components p91/p84, STAT1)

Cat. Num. AAA626209

• Gene Names: SOAT1; ACAT; SOAT; STAT; ACACT; ACAT1; ACAT-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: STAT 1; Polyclonal Antibody; phosphorylated (Ser727) (Signal Transducer and Activator of Transcription 1-alpha/beta; Transcription Factor ISGF-3 Components p91/p84; STAT1); Anti -STAT 1; anti-SOAT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human STAT 1 when phosphorylated at serine 727. Species Cross-Reactivity: mouse, rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-SOAT1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot:1:500-1:1,000; Immunohistochemistry:1:50-1:100
• Immunogen: Synthetic phosphopeptide corresponding to human STAT 1 around the phosphorylation site of serine 727 (P-M-SP-P-E).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-SOAT1 antibody

STATs (signal transducers and activators of transcription) were originally discovered as two proteins (STAT1 and STAT2) which were involved in interferon alpha (IFN alpha) and IFN gamma signal transduction. Since then, several additional STAT proteins have been identified (STAT3, 4, 5a, 5b, and 6). STATs undergo tyrosine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. In some cases this process is mediated by JAK Kinases (Janus Kinases 1, 2, and 3). For maximum activation of these proteins, phosphorylation at specific tyrosine and serine residues may be required in STAT1 alpha, 3, 4, and 5. Specific functions of the STAT proteins are poorly defined at this time. They are thought to be involved in a variety of systems including antiviral responses and cell transformation. STAT1 deficient knock-out mice are unable to induce IFN regulated genes and are extremely susceptible to viral diseases. After phosphorylation, STAT1 and STAT2 form heterodimers that function as more potent inducers of transcription than the STAT1 homodimer.

STAT1 is known to associate with the transcription factors p48, Sp1, and p300. STAT1 is activated by peptide hormones, growth factors and cytokines, particularly cytokines of the interferon family. STAT1 alpha and STAT1 beta isoforms arise by alternative splicing of a single gene. The only difference between the two proteins is that STAT1 beta lacks 38 carboxy terminal amino acids. Contained within these 38 terminal aa of STAT1 alpha is a critical serine residue (Ser 727) whose phosphorylation is required for maximal transcription activity induced by IFN gamma.

Product Categories/Family for anti-SOAT1 antibody

Antibodies; Abs to Transcription Factors; STAT

NCBI and Uniprot Product Information

• NCBI GI #: 49533617
• NCBI GeneID: 6646
• NCBI Accession #: NP_003092.4
• NCBI GenBank Nucleotide #: NM_003101.5
• UniProt Accession #: P35610; Q5T0X4; Q8N1E4; A6NC40; A8K3P4; A9Z1V7; B4DU95
• Molecular Weight: 64,735 Da
• NCBI Official Full Name: sterol O-acyltransferase 1 isoform 1
• NCBI Official Synonym Full Names: sterol O-acyltransferase 1
• NCBI Official Symbol: SOAT1
• NCBI Official Synonym Symbols: ACAT; SOAT; STAT; ACACT; ACAT1; ACAT-1
• NCBI Protein Information: sterol O-acyltransferase 1; acyl-coenzyme A:cholesterol acyltransferase 1; sterol O-acyltransferase (acyl-Coenzyme A: cholesterol acyltransferase) 1
• UniProt Protein Name: Sterol O-acyltransferase 1
• Protein Family: Sterol O-acyltransferase
• UniProt Gene Name: SOAT1
• UniProt Synonym Gene Names: ACACT; ACACT1; ACAT; ACAT1; SOAT; STAT; ACAT-1
• UniProt Entry Name: SOAT1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the acyltransferase family. It is located in the endoplasmic reticulum, and catalyzes the formation of fatty acid-cholesterol esters. This gene has been implicated in the formation of beta-amyloid and atherosclerotic plaques by controlling the equilibrium between free cholesterol and cytoplasmic cholesteryl esters. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

SOAT1: Catalyzes the formation of fatty acid-cholesterol esters. Plays a role in lipoprotein assembly and dietary cholesterol absorption. In addition to its acyltransferase activity, it may act as a ligase. Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.

Protein type: Membrane protein, integral; Lipid Metabolism - steroid biosynthesis; Membrane protein, multi-pass; EC 2.3.1.26; Transferase

Chromosomal Location of Human Ortholog: 1q25

Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane

Molecular Function: protein binding; acyl-CoA binding; sterol O-acyltransferase activity; cholesterol binding

Biological Process: cholesterol metabolic process; cholesterol homeostasis; cholesterol efflux; positive regulation of amyloid precursor protein biosynthetic process