Western Blot (WB)
(Western blot analysis of lysate from SW480 cell line,using DNAJB11 Antibody (N-term). MBS9208581 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.)
Rabbit DNAJB11 Polyclonal Antibody | anti-DNAJB11 antibody
DNAJB11 Antibody (N-term)
Western Blot (WB)
(Western blot analysis of lysate from SW480 cell line,using DNAJB11 Antibody (N-term). MBS9208581 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.)
Western Blot (WB)
(Western blot analysis of lysate from SW480 cell line,using DNAJB11 Antibody (N-term). MBS9208581 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.)
Immunohistochemistry (IHC)
(DNAJB11 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DNAJB11 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(DNAJB11 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DNAJB11 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
family of proteins, which regulate molecular chaperone activity by
stimulating ATPase activity. DNAJ proteins may have up to 3
distinct domains: a conserved 70-amino acid J domain, usually at
the N terminus; a glycine/phenylalanine (G/F)-rich region; and a
C-terminal cysteine-rich region (Ohtsuka and Hata, 2000 [PubMed
11147971]).
Wen, K.W., et al. Oncogene 29(24):3532-3544(2010)
Bernal-Bayard, J., et al. J. Biol. Chem. 285(21):16360-16368(2010)
Vembar, S.S., et al. J. Biol. Chem. 284(47):32462-32471(2009)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a soluble glycoprotein of the endoplasmic reticulum (ER) lumen that functions as a co-chaperone of binding immunoglobulin protein, a 70 kilodalton heat shock protein chaperone required for the proper folding and assembly of proteins in the ER. The encoded protein contains a highly conserved J domain of about 70 amino acids with a characteristic His-Pro-Asp (HPD) motif and may regulate the activity of binding immunoglobulin protein by stimulating ATPase activity. [provided by RefSeq, Mar 2014]
Uniprot Description
DNAJB11: Serves as a co-chaperone for HSPA5. Binds directly to both unfolded proteins that are substrates for ERAD and nascent unfolded peptide chains, but dissociates from the HSPA5-unfolded protein complex before folding is completed. May help recruiting HSPA5 and other chaperones to the substrate. Stimulates HSPA5 ATPase activity.
Protein type: Endoplasmic reticulum; Secreted; RNA processing; Secreted, signal peptide; Chaperone
Chromosomal Location of Human Ortholog: 3q27.3
Cellular Component: membrane; endoplasmic reticulum; endoplasmic reticulum lumen; nucleus
Molecular Function: protein binding; unfolded protein binding
Biological Process: cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; protein folding; unfolded protein response; mRNA modification
Research Articles on DNAJB11
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Product Notes
The DNAJB11 dnajb11 (Catalog #AAA9208581) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 63-92. The DNAJB11 Antibody (N-term) reacts with Human (Predicted Reactivity: Bovine, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's DNAJB11 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). WB~~1:1000. Researchers should empirically determine the suitability of the DNAJB11 dnajb11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNAJB11, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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