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Western Blot (WB) (Anti-ST2 rabbit polyclonal antibody at 1:500 dilutionLane A: Daudi Whole Cell LysateLane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:29 kDaObserved band size:29 kDa)

Rabbit anti-Mouse ST2/IL-1 RL1 Polyclonal Antibody | anti-IL1RL1 antibody

Anti-ST2/IL-1 RL1 Antibody, Rabbit Polyclonal

Gene Names
Il1rl1; T1; St2; DER4; Ly84; ST2L; Fit-1; T1/ST2; St2-rs1
Reactivity
Mouse
Applications
Western Blot, ELISA
Purity
Protein A & Antigen Affinity
Synonyms
ST2/IL-1 RL1; Polyclonal Antibody; Anti-ST2/IL-1 RL1 Antibody; Rabbit Polyclonal; IL1RL1/IL?1 R4 Antibody; Rabbit PAb; Antigen Affinity Purified; interleukin 1 receptor-like 1; Anti-DER4 Antibody; Anti-Fit-1 Antibody; Anti-Ly84 Antibody; Anti-St2 Antibody; Anti-St2-rs1 Antibody; Anti-ST2L Antibody; Anti-T1 Antibody; Anti-T1/ST2 Antibody; anti-IL1RL1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse IL1RL1/IL?1 R4
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-IL1RL1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
ELISA: 1:25,000-1:50,000
Immunogen
Recombinant Mouse IL1RL1/IL-1 R4 Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Mouse IL1RL1/IL?1 R4 (rM IL1RL1/IL?1 R4). IL1RL1/IL?1 R4 specific IgG was purified by Mouse IL1RL1/IL?1 R4 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-ST2 rabbit polyclonal antibody at 1:500 dilutionLane A: Daudi Whole Cell LysateLane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:29 kDaObserved band size:29 kDa)

Western Blot (WB) (Anti-ST2 rabbit polyclonal antibody at 1:500 dilutionLane A: Daudi Whole Cell LysateLane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:29 kDaObserved band size:29 kDa)
Related Product Information for anti-IL1RL1 antibody
IL-1 receptor-like 1 (IL1RL1) is a membrane receptor involved in TH2 inflammatory responses and eosinophilia. It has previously been described that levels of the interleukin-1 like 1 (IL1RL1) protein can be used to identifies cardiovascular disease and determine the prognosis for a patient with cardiovascular disease. The ligand for IL1RL1 has been described and named IL-33. Mutants in IL1RL1 have been associated with blood eosinophil counts in a genome-wide association study and with asthma in family-based and case-control studies. As an important mediator involved in many immune and inflammatory responses, this cytokine has been implicated as a regulator of both the development and effector phases of type 2 helper T cell responses, and as a negative feedback modulator of macrophage proinflammatory function. IL33 is a specific ligand of ST2L and induces the production of Th2 cytokines.
Product Categories/Family for anti-IL1RL1 antibody
References
Savenije OE, et al. (2011) Interleukin-1 receptor-like 1 polymorphisms are associated with serum IL1RL1-a, eosinophils, and asthma in childhood. J Allergy Clin Immunol. 127(3): 750-6.Li H, et al. (2000) The cloning and nucleotide sequence of human ST2L cDNA. Genomics. 67(3): 284-90.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
interleukin-1 receptor-like 1 isoform a
NCBI Official Synonym Full Names
interleukin 1 receptor-like 1
NCBI Official Symbol
Il1rl1
NCBI Official Synonym Symbols
T1; St2; DER4; Ly84; ST2L; Fit-1; T1/ST2; St2-rs1
NCBI Protein Information
interleukin-1 receptor-like 1; lymphocyte antigen 84
UniProt Protein Name
Interleukin-1 receptor-like 1
Protein Family
UniProt Gene Name
Il1rl1
UniProt Synonym Gene Names
Ly84; St2; Ste2
UniProt Entry Name
ILRL1_MOUSE

Uniprot Description

IL1RL1: receptor for IL33. Its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of ERK1 and/or ERK2, p38-alpha, and JNK1. Apparently promotes the activation and differentiation of T-helper 2 cells. Belongs to the interleukin-1 receptor family. Highly expressed in kidney, lung, placenta, stomach, skeletal muscle, colon and small intestine. Three isoforms of the human protein are produced by alternative splicing. Isoform A is prevalently expressed in the lung, testis, placenta, stomach and colon. Isoform B is more abundant in the brain, kidney and the liver. Isoform C is not detected in brain, heart, liver, kidney and skeletal muscle.

Protein type: Membrane protein, integral; Receptor, cytokine

Cellular Component: proteinaceous extracellular matrix; extracellular space; cell surface; membrane; integral to membrane; plasma membrane; extracellular region; external side of plasma membrane

Molecular Function: interleukin-1 receptor activity

Biological Process: negative regulation of cell proliferation; negative regulation of T-helper 1 type immune response; negative regulation of interferon-gamma production; negative regulation of I-kappaB kinase/NF-kappaB cascade; signal transduction; positive regulation of interleukin-5 production; positive regulation of macrophage activation; positive regulation of inflammatory response

Research Articles on IL1RL1

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Product Notes

The IL1RL1 il1rl1 (Catalog #AAA8112356) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ST2/IL-1 RL1 Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ST2/IL-1 RL1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:1000 ELISA: 1:25,000-1:50,000. Researchers should empirically determine the suitability of the IL1RL1 il1rl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ST2/IL-1 RL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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