Western Blot (WB)
(SSX4 Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the SSX4 antibody detected the SSX4 protein (arrow).)
Rabbit anti-Human SSX4 Polyclonal Antibody
SSX4, ID (SSX4, SSX4A, Protein SSX4, Cancer/testis antigen 5.4)
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
SSX4; Polyclonal Antibody; ID (SSX4; SSX4A; Protein SSX4; Cancer/testis antigen 5.4); Anti -SSX4; anti-SSX4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-SSX4 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunogen
SSX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-97 amino acids from the Central region of human SSX4.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(SSX4 Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the SSX4 antibody detected the SSX4 protein (arrow).)
Western Blot (WB)
(SSX4 Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the SSX4 antibody detected the SSX4 protein (arrow).)
Related Product Information for anti-SSX4 antibody
The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneously humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. SSX1, SSX2 and SSX4 genes have been involved in the t(X;18) translocation characteristically found in all synovial sarcomas. This translocation results in the fusion of the synovial sarcoma translocation gene on chromosome 18 to one of the SSX genes on chromosome X. Chromosome Xp11 contains a segmental duplication resulting in two identical copies of synovial sarcoma, X breakpoint 4, SSX4 and SSX4B, in tail-to-tail orientation. This gene, SSX4B, represents the more centromeric copy. Two transcript variants encoding distinct isoforms have been identified for this gene.
Product Categories/Family for anti-SSX4 antibody
NCBI and Uniprot Product Information
Uniprot Description
SSX4: Could act as a modulator of transcription. Belongs to the SSX family.
Protein type: Cancer Testis Antigen (CTA)
Chromosomal Location of Human Ortholog: Xp11.23
Cellular Component: nucleus
Molecular Function: nucleic acid binding
Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent
Similar Products
Product Notes
The SSX4 (Catalog #AAA6006003) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SSX4, ID (SSX4, SSX4A, Protein SSX4, Cancer/testis antigen 5.4) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SSX4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the SSX4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SSX4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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