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Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using GOLGB1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)
Rabbit anti-Human GOLGB1 Polyclonal Antibody | anti-GOLGB1 antibody
GOLGB1 Rabbit pAb
Gene Names
GOLGB1; GCP; GCP372; GOLIM1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Synonyms
GOLGB1; Polyclonal Antibody; GOLGB1 Rabbit pAb; GCP; GCP372; GOLIM1; anti-GOLGB1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Applicable Applications for anti-GOLGB1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:50-1:200
IHC: 1:50-1:200
IF: 1:50-1:200
Immunogen
Recombinant protein of human GOLGB1.
Positive Samples
HeLa
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using GOLGB1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)
Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using GOLGB1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human colon carcinoma using GOLGB1 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human colon carcinoma using GOLGB1 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human esophageal cancer using GOLGB1 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human esophageal cancer using GOLGB1 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U2OS cells using GOLGB1 Rabbit pAb at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U2OS cells using GOLGB1 Rabbit pAb at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
377,137 Da
NCBI Official Full Name
GOLGB1 protein, partial
NCBI Official Synonym Full Names
golgin B1
NCBI Official Symbol
GOLGB1
NCBI Official Synonym Symbols
GCP; GCP372; GOLIM1
NCBI Protein Information
golgin subfamily B member 1
UniProt Protein Name
Golgin subfamily B member 1
Protein Family
UniProt Gene Name
GOLGB1
UniProt Synonym Gene Names
GCP372
Uniprot Description
May participate in forming intercisternal cross-bridges of the Golgi complex.
Research Articles on GOLGB1
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Product Notes
The GOLGB1 golgb1 (Catalog #AAA9143673) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GOLGB1 Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GOLGB1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC: 1:50-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the GOLGB1 golgb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GOLGB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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