Rabbit anti-Human SSRP1 Polyclonal Antibody | anti-SSRP1 antibody

SSRP1 Antibody

Cat. Num. AAA7045164

• Gene Names: SSRP1; FACT; T160; FACT80
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: SSRP1; Polyclonal Antibody; SSRP1 Antibody; FACT complex subunit SSRP1; Chromatin-specific transcription elongation factor 80 kDa subunit; Facilitates chromatin transcription complex 80 kD; FACT80; anti-SSRP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 709

• Applicable Applications for anti-SSRP1 antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Species: Human
• Immunogen: Recombinant human FACT complex subunit SSRP1 protein
• Conjugate: Non-conjugated
• Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-5911 / sc-5909 / sc-25382
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Immunofluorescence (IF)

(Immunofluorescent analysis of HepG-2 cells using MBS7045164 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Western Blot (WB)

(Western BlotPositive WB detected in:Hela whole cell lysate,HL60 whole cell lysate,K562 whole cell lysate,A549 whole cell lysateAll lanes: SSRP1 antibody at 3.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 82 kDaObserved band size: 82 kDa)

Related Product Information for anti-SSRP1 antibody

Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Binds specifically to double-stranded DNA and at low levels to DNA modified by the antitumor agent cisplatin. May potentiate cisplatin-induced cell death by blocking replication and repair of modified DNA. Also acts as a transcriptional coactivator for p63/TP63.

NCBI and Uniprot Product Information

• NCBI GI #: 4507241
• NCBI GeneID: 6749
• NCBI Accession #: NP_003137.1
• NCBI GenBank Nucleotide #: NM_003146.2
• UniProt Accession #: Q08945; Q5BJG8
• Molecular Weight: 81,075 Da
• NCBI Official Full Name: FACT complex subunit SSRP1
• NCBI Official Synonym Full Names: structure specific recognition protein 1
• NCBI Official Symbol: SSRP1
• NCBI Official Synonym Symbols: FACT; T160; FACT80
• NCBI Protein Information: FACT complex subunit SSRP1
• UniProt Protein Name: FACT complex subunit SSRP1
• UniProt Gene Name: SSRP1
• UniProt Synonym Gene Names: FACT80; FACT 80 kDa subunit; FACTp80; hSSRP1
• UniProt Entry Name: SSRP1_HUMAN

NCBI Description

The protein encoded by this gene is a subunit of a heterodimer that, along with SUPT16H, forms chromatin transcriptional elongation factor FACT. FACT interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT and cisplatin-damaged DNA may be crucial to the anticancer mechanism of cisplatin. This encoded protein contains a high mobility group box which most likely constitutes the structure recognition element for cisplatin-modified DNA. This protein also functions as a co-activator of the transcriptional activator p63. An alternatively spliced transcript variant of this gene has been described, but its full-length nature is not known. [provided by RefSeq, Jul 2008]

Uniprot Description

SSRP1: a subunit of the chromatin transcriptional elongation factor FACT. FACT interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT and cisplatin-damaged DNA may be crucial to the anticancer mechanism of cisplatin. This encoded protein contains a high mobility group box which most likely constitutes the structure recognition element for cisplatin-modified DNA. This protein also functions as a co-activator of the transcriptional activator p63.

Protein type: Nucleolus; Transcription, coactivator/corepressor; RNA-binding; Transcription initiation complex

Chromosomal Location of Human Ortholog: 11q12

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function: protein binding

Biological Process: RNA elongation from RNA polymerase II promoter; transcription from RNA polymerase II promoter