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Western Blot (WB) (CCT8 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CCT8 antibody detected the CCT8 protein (arrow).)

Rabbit anti-Human CCT8 Polyclonal Antibody | anti-CCT8 antibody

CCT8, CT (CCT8, C21orf112, CCTQ, KIAA0002, T-complex protein 1 subunit theta, CCT-theta, Renal carcinoma antigen NY-REN-15) (AP)

Gene Names
CCT8; Cctq; PRED71; D21S246; C21orf112
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
CCT8; Polyclonal Antibody; CT (CCT8; C21orf112; CCTQ; KIAA0002; T-complex protein 1 subunit theta; CCT-theta; Renal carcinoma antigen NY-REN-15) (AP); Renal carcinoma antigen NY-REN-15; anti-CCT8 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-CCT8 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:10-50
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
CCT8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 518-548 amino acids from the C-terminal region of human CCT8.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CCT8 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CCT8 antibody detected the CCT8 protein (arrow).)

Western Blot (WB) (CCT8 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CCT8 antibody detected the CCT8 protein (arrow).)

Immunohistochemistry (IHC)

(CCT8 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CCT8 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (CCT8 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CCT8 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CCT8 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (CCT8 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-CCT8 antibody
Molecular chaperone; CCT8 assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
Product Categories/Family for anti-CCT8 antibody
References
Mukherjee, K., et al. BMC Evol. Biol. 10, 64 (2010): Zebol, J.R., et al. Int. J. Biochem. Cell Biol. 41(4):822-827(2009). Kim, S.C., et al. Mol. Cell 23(4):607-618(2006). Hu, Y.H., et al. BMC Genomics 7, 155 (2006): Wang, L., et al. J. Mol. Med. 83(10):812-821(2005).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
59,621 Da
NCBI Official Full Name
T-complex protein 1 subunit theta
NCBI Official Synonym Full Names
chaperonin containing TCP1, subunit 8 (theta)
NCBI Official Symbol
CCT8
NCBI Official Synonym Symbols
Cctq; PRED71; D21S246; C21orf112
NCBI Protein Information
T-complex protein 1 subunit theta; CCT-theta; TCP-1-theta; renal carcinoma antigen NY-REN-15; T-complex protein 1, theta subunit
UniProt Protein Name
T-complex protein 1 subunit theta
UniProt Gene Name
CCT8
UniProt Synonym Gene Names
C21orf112; CCTQ; KIAA0002; TCP-1-theta
UniProt Entry Name
TCPQ_HUMAN

NCBI Description

This gene encodes the theta subunit of the CCT chaperonin, which is abundant in the eukaryotic cytosol and may be involved in the transport and assembly of newly synthesized proteins. Alternative splicing results in multiple transcript variants of this gene. A pseudogene related to this gene is located on chromosome 1. [provided by RefSeq, Sep 2013]

Uniprot Description

CCT-theta: a chaperone of the TCP-1 chaperonin family. A part of a hetero-oligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.

Protein type: Chaperone; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 21q22.11

Cellular Component: chaperonin-containing T-complex; microtubule; centrosome; intermediate filament cytoskeleton; cytoplasm; cytosol

Molecular Function: protein binding; unfolded protein binding; ATPase activity, coupled; ATP binding

Biological Process: 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; binding of sperm to zona pellucida; pore complex biogenesis

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Product Notes

The CCT8 cct8 (Catalog #AAA6282685) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CCT8, CT (CCT8, C21orf112, CCTQ, KIAA0002, T-complex protein 1 subunit theta, CCT-theta, Renal carcinoma antigen NY-REN-15) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CCT8 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:10-50 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CCT8 cct8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCT8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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