Typical Testing Data/Standard Curve (for reference only)
Canine Junction plakoglobin (JUP) ELISA Kit | JUP elisa kit
Canine Junction plakoglobin (JUP) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: JUP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-JUP antibody and an JUP-HRP conjugate. The assay sample and buffer are incubated together with JUP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the JUP concentration since JUP from samples and JUP-HRP conjugate compete for the anti-JUP antibody binding site. Since the number of sites is limited, as more sites are occupied by JUP from the sample, fewer sites are left to bind JUP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The JUP concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a major cytoplasmic protein which is the only known constituent common to submembranous plaques of both desmosomes and intermediate junctions. This protein forms distinct complexes with cadherins and desmosomal cadherins and is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat. Mutation in this gene has been associated with Naxos disease. Alternative splicing occurs in this gene; however, not all transcripts have been fully described. [provided by RefSeq, Jul 2008]
Uniprot Description
CTNNG: Common junctional plaque protein. The membrane- associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE- cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton. Homodimer. Component of an E-cadherin/ catenin adhesion complex composed of at least E-cadherin/CDH1 and gamma- catenin/JUP, and possibly alpha-catenin/CTNNA1; the complex is located to adherens junctions. The stable association of CTNNA1 is controversial as CTNNA1 was shown not to bind to F-actin when assembled in the complex. Interacts with MUC1. Interacts with CAV1. Interacts with PTPRJ. Interacts with DSC2. Belongs to the beta-catenin family.
Protein type: Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 17q21
Cellular Component: desmosome; internal side of plasma membrane; focal adhesion; apicolateral plasma membrane; intermediate filament; fascia adherens; intercellular junction; catenin complex; zonula adherens; Z disc; cytosol; actin cytoskeleton; cell-cell adherens junction; cytoskeleton; hemidesmosome; cytoplasm; plasma membrane; nucleus; lateral plasma membrane
Molecular Function: signal transducer activity; protein binding; protein homodimerization activity; structural constituent of cell wall; cadherin binding; transcription coactivator activity; structural molecule activity; protein kinase binding; protein phosphatase binding; alpha-catenin binding; nuclear hormone receptor binding
Biological Process: skin development; intercellular junction assembly and maintenance; cell migration; cell-cell adhesion; positive regulation of protein import into nucleus; protein heterooligomerization; cytoskeletal anchoring; regulation of cell fate specification; detection of mechanical stimulus; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; vascular endothelial growth factor receptor signaling pathway; regulation of cell proliferation
Disease: Arrhythmogenic Right Ventricular Dysplasia, Familial, 12; Naxos Disease
Research Articles on JUP
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Product Notes
The Canine JUP jup (Catalog #AAA7219571) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7219571 ELISA Kit recognizes Canine JUP. It is sometimes possible for the material contained within the vial of "Junction plakoglobin (JUP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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