IL3 active protein

IL3 protein

Cat. Num. AAA5304090

• Gene Names: IL3; IL-3; MCGF; MULTI-CSF
• Applications: Flow Cytometry, Functional Assay
• Purity: > 95% pure
• Synonyms: IL3; IL3 protein; P-cell-stimulating factor protein; Multipotential colony-stimulating factor protein; IL 3; MCGF protein; IL-3 protein; Interleukin-3 protein; Mast cell growth factor protein; IL 3 protein; IL-3; Hematopoietic growth factor protein; IL3 active protein

• Host: Human
• Specificity: Mouse
• Purity/Purification: > 95% pure
• Form/Format: Supplied as a sterile liquid with 20mM phosphate nuffer, 0.15MNaCl, pH 7.0, 0.22 um filtered.
• Concentration: 200 ug/mL (varies by lot)
• Sequence Length: 152

• Applicable Applications for IL3 active protein: Flow Cytometry (FC/FACS)
• Application Notes: FC: 5 ul (0.125 ug)/test; ; A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
• Protein Type: Recombinant
• Biological Significance: Interleukin 3, also called Mast Cell Growth Factor, Multi-Colony Stimulating Factor, Eosinophil-CSF, is produced by activated T cells, mast cells and eosinophils. IL-3 is a hematopoietic growth factor that stimulates colony formation of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell and monocytic lineages. Most of these functions are enhanced or dependent on co-stimulation with other cytokines. Human and mouse IL-3 share only 29% sequence identity at the amino acid level. The bioactivity of IL-3 is highly species specific. Human IL-3 has no activity on mouse cells.
• Bioactivity: The ED50 measured in a TF-1 proliferation assay is typically less than 0.15 ng/ml, corresponding to a specific activity of greater than 7x10^6 Units/mg.
• Endotoxin Levels: Less than 0.01 ng/ug cytokine as determined by the LAL assay.
• Expression System: E Coli
• Preparation and Storage: Store at -70 degree C or less.

Functional Assay (FA)

(Proliferation of the TF1 cell line in response to Human IL3 protein)

Related Product Information for IL3 active protein

Purified recombinant Human IL3 protein

Product Categories/Family for IL3 active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 28416915
• NCBI GeneID: 3562
• NCBI Accession #: NP_000579.2
• NCBI GenBank Nucleotide #: NM_000588.3
• Molecular Weight: 15 kDa
• NCBI Official Full Name: interleukin-3
• NCBI Official Synonym Full Names: interleukin 3
• NCBI Official Symbol: IL3
• NCBI Official Synonym Symbols: IL-3; MCGF; MULTI-CSF
• NCBI Protein Information: interleukin-3
• UniProt Protein Name: Interleukin-3
• Protein Family: Interleukin
• UniProt Gene Name: IL3
• UniProt Synonym Gene Names: IL-3; MCGF
• UniProt Entry Name: IL3_HUMAN

NCBI Description

The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. [provided by RefSeq, Jul 2008]

Uniprot Description

IL3: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. Belongs to the IL-3 family.

Protein type: Secreted; Apoptosis; Cytokine; Cell cycle regulation; Secreted, signal peptide; Oncoprotein

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space; extracellular region

Molecular Function: interleukin-3 receptor binding; growth factor activity; cytokine activity

Biological Process: nervous system development; positive regulation of peptidyl-tyrosine phosphorylation; cell-cell signaling; positive regulation of tyrosine phosphorylation of Stat5 protein; embryonic hemopoiesis; cytokine and chemokine mediated signaling pathway; positive regulation of cell proliferation; immune response; positive regulation of myeloid leukocyte differentiation; positive regulation of DNA replication