Rabbit Rho A/B/C Monoclonal Antibody | anti-RhoA/B/C antibody

Rho A/B/C Rabbit Monoclonal Antibody

Cat. Num. AAA3014073

• Gene Names: RHOC; H9; ARH9; ARHC; RHOH9
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Protein A affinity purified
• Synonyms: Rho A/B/C; Monoclonal Antibody; Rho A/B/C Rabbit Monoclonal Antibody; Rho-related GTP-binding protein RhoC; Rho cDNA clone 9; RHOC; ARH9; ARHC; Transforming protein RhoA; Rho cDNA clone 12; RHOA; ARH12; ARHA; RHO12; Rho-related GTP-binding protein RhoB; Rho cDNA clone 6; RHOB; ARH6; ARHB; anti-RhoA/B/C antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Specificity: This antibody detects endogenous levels of Rho A/B/C and does not cross-react with related proteins.
• Purity/Purification: Protein A affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 193

• Applicable Applications for anti-RhoA/B/C antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000-1:2000; ICC: 1:50-1:20; IF: 1:50-1:200; IHC: 1:200-1:500; FC/FACS: 1:50-1:100
• Immunogen: Recombinant antibody.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Rho A/B/C Rabbit mAb at 1:1000 dilutionLane1:HepG2 whole cell lysateLane2:Hela whole cell lysateLane3:Jurkat whole cell lysate)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-Rho A/B/C antibody. Counter stained with hematoxylin.)

Immunocytochemistry (ICC)

(ICC staining Rho A/B/C in Ags cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Rho A/B/C antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Rho A/B/C antibody. Counter stained with hematoxylin.)

Immunocytochemistry (ICC)

(ICC staining Rho A/B/C in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.)

Immunocytochemistry (ICC)

(ICC staining Rho A/B/C in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of HepG2 cells with Rho A+B+C antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

Related Product Information for anti-RhoA/B/C antibody

The Ras p21 family of guanine nucleotide proteins has been widely studied in view of its apparent role in signal transduction pathways and high frequency of mutations in human malignancies. It is now clear, however, that the Ras proteins (H-, K- and N-Ras p21) are members of a much larger superfamily of related proteins. Six members of this family, Rap 1A, Rap 1B, Rap 2, R-Ras, Ral A and Ral B, exhibit approximately 50% amino acid homology to Ras. The six mammalian Rho proteins (Rho A, B, C, G, 7 and 8) are approximately 30% homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy-terminal CAAX sequence (C, cysteine; A, aliphatic amino acid; X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function.

NCBI and Uniprot Product Information

• NCBI GI #: 111494248
• NCBI GeneID: 389
• NCBI Accession #: NP_001036143.1
• NCBI GenBank Nucleotide #: NP_001036143.1
• UniProt Accession #: P08134; P61586; P62745
• Molecular Weight: ~ 24kDa
• NCBI Official Full Name: rho-related GTP-binding protein RhoC
• NCBI Official Synonym Full Names: ras homolog family member C
• NCBI Official Symbol: RHOC
• NCBI Official Synonym Symbols: H9; ARH9; ARHC; RHOH9
• NCBI Protein Information: rho-related GTP-binding protein RhoC
• UniProt Protein Name: Rho-related GTP-binding protein RhoC
• UniProt Gene Name: RHOC
• UniProt Synonym Gene Names: ARH9; ARHC; h9
• UniProt Entry Name: RHOC_HUMAN

NCBI Description

This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]