Rabbit anti-Human ST14 / Matriptase Polyclonal Antibody | anti-ST14 antibody

Rabbit Polyclonal (IgG) to Human ST14 / Matriptase

Cat. Num. AAA243381

• Gene Names: ST14; HAI; MTSP1; SNC19; ARCI11; MT-SP1; PRSS14; TADG15; TMPRSS14
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: ST14 / Matriptase; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human ST14 / Matriptase; Anti-ST14 / Matriptase Antibody (aa10-59) IHC-plus; ST14; Epithin; HAI; Prostamin; PRSS14; TADG15; Matriptase; MT-SP1; SNC19; TMPRSS14; MTSP1; Serine protease 14; Serine protease TADG-15; TADG-15; Human ST14; anti-ST14 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ST14 Antibody detects endogenous levels of total ST14 protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 855

• Applicable Applications for anti-ST14 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:10000), IF (1:100 - 1:500), IHC-P (10 ug/ml), WB (1:500 - 1:1000)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human ST14 (aa10-59).
• Immunogen: ST14 / Matriptase antibody was raised against synthetic peptide from human ST14 (aa10-59).
• Antigen Modification: aa10-59
• Preparation and Storage: Store at -20 degree C for up to one year.

Immunohistochemistry (IHC)

(Anti-ST14 / Matriptase antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-ST14 / Matriptase antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of A549 cells, using ST14 Antibody. The picture on the right is treated with the synthesized peptide.)

Western Blot (WB)

(Western blot of extracts from A549 cells, using ST14 Antibody. The lane on the right is treated with the synthesized peptide.)

Product Categories/Family for anti-ST14 antibody

Family: Protease; Subfamily: Serine S1

NCBI and Uniprot Product Information

• NCBI GI #: 11415040
• NCBI GeneID: 6768
• NCBI Accession #: NP_068813.1
• NCBI GenBank Nucleotide #: NM_021978.3
• UniProt Accession #: Q9Y5Y6; Q9BS01; Q9H3S0; Q9HB36; Q9HCA3
• Molecular Weight: 94,770 Da
• NCBI Official Full Name: suppressor of tumorigenicity 14 protein
• NCBI Official Synonym Full Names: suppression of tumorigenicity 14 (colon carcinoma)
• NCBI Official Symbol: ST14
• NCBI Official Synonym Symbols: HAI; MTSP1; SNC19; ARCI11; MT-SP1; PRSS14; TADG15; TMPRSS14
• NCBI Protein Information: suppressor of tumorigenicity 14 protein; prostamin; serine protease 14; serine protease TADG-15; membrane-type serine protease 1; tumor associated differentially expressed gene 15 protein; tumor-associated differentially-expressed gene 15 protein; suppression of tumorigenicity 14 (colon carcinoma, matriptase, epithin)
• UniProt Protein Name: Suppressor of tumorigenicity 14 protein
• Protein Family: Suppressor of tumorigenicity 14 protein
• UniProt Gene Name: ST14
• UniProt Synonym Gene Names: PRSS14; SNC19; TADG15; MT-SP1
• UniProt Entry Name: ST14_HUMAN

NCBI Description

The protein encoded by this gene is an epithelial-derived, integral membrane serine protease. This protease forms a complex with the Kunitz-type serine protease inhibitor, HAI-1, and is found to be activated by sphingosine 1-phosphate. This protease has been shown to cleave and activate hepatocyte growth factor/scattering factor, and urokinase plasminogen activator, which suggest the function of this protease as an epithelial membrane activator for other proteases and latent growth factors. The expression of this protease has been associated with breast, colon, prostate, and ovarian tumors, which implicates its role in cancer invasion, and metastasis. [provided by RefSeq, Jul 2008]

Uniprot Description

ST14: Degrades extracellular matrix. Proposed to play a role in breast cancer invasion and metastasis. Exhibits trypsin-like activity as defined by cleavage of synthetic substrates with Arg or Lys as the P1 site. Defects in ST14 are a cause of ichthyosis autosomal recessive with hypotrichosis (ARIH). ARIH is a skin disorder characterized by congenital ichthyosis associated with the presence of less than the normal amount of hair. Belongs to the peptidase S1 family.

Protein type: EC 3.4.21.109; Membrane protein, integral; Protease

Chromosomal Location of Human Ortholog: 11q24-q25

Cellular Component: extrinsic to plasma membrane; extracellular space; basolateral plasma membrane; integral to plasma membrane; plasma membrane

Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: keratinocyte differentiation; proteolysis

Disease: Ichthyosis, Congenital, Autosomal Recessive 11