Rabbit anti-Human, Mouse Sprouty 2 Polyclonal Antibody | anti-Spry2 antibody

Sprouty 2 (Sprouty-2, Sprouty Homolog 2 (Drosophila), Spry 2, Spry2, Spry-2, hSPRY2, MGC23039) (MaxLight 650)

Cat. Num. AAA6500576

• Gene Names: SPRY2; IGAN3; hSPRY2
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Sprouty 2; Polyclonal Antibody; Sprouty 2 (Sprouty-2; Sprouty Homolog 2 (Drosophila); Spry 2; Spry2; Spry-2; hSPRY2; MGC23039) (MaxLight 650); anti-Spry2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes Sprouty2, Mr ~35-40kD. A non-specific protein was also detected, Mr ~70kD. Species Crossreactivity: Human and mouse. Crossreactivity with rat predicted, based on sequence homology.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.
• Sequence Length: 2286

• Applicable Applications for anti-Spry2 antibody: Western Blot (WB)
• Application Notes: WB: 0.1-1ug/ml detects Sprouty2 in RIPA lysates from HeLa and 3T3/A31 cells.; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated, synthetic peptide corresponding to aa26-39 (C-GEPDPRDALTQQVH) of human Sprouty2, with an N-terminal cysteine added for conjugation purposes. The immunizing sequence is identical in rat and mouse.
• Conjugate: MaxLight650
• Included Positive Antigen Control: MBS657179: 3T3/A31 lysate. Add 2.5ul of 2-mercaptoethanol per 100ul of lysate and boil for 5 minutes to reduce the preparation. Load 10ug of reduced lysate per lane for minigels.
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Testing Data

(3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Sprouty2 (0.2ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Sprouty2 (35-40kD).)

Related Product Information for anti-Spry2 antibody

Cysteine added for conjugation purposes. The immunizing sequence is identical in rat and mouse.

Product Categories/Family for anti-Spry2 antibody

Antibodies; Sprouty

References

1. Hacohen, N., et al., Cell 92: 253-263 (1998). 2. Hanafusa, H., et al., Nature Cell Biol. 4: 850-858 (2002). 3. Wong, E.S., et al., J. Biol. Chem. 276: 5866-5875 (2001). 4. Yusoff, P., et al., J. Biol. Chem. 277: 3195-3201 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 1519243998
• NCBI GeneID: 10253
• NCBI Accession #: NM_005842.4
• NCBI GenBank Nucleotide #: NM_005842
• UniProt Accession #: O43597
• Molecular Weight: 3540
• NCBI Official Full Name: Homo sapiens sprouty RTK signaling antagonist 2 (SPRY2), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: sprouty RTK signaling antagonist 2
• NCBI Official Symbol: SPRY2
• NCBI Official Synonym Symbols: IGAN3; hSPRY2
• NCBI Protein Information: protein sprouty homolog 2
• UniProt Protein Name: Protein sprouty homolog 2
• Protein Family: Protein sprouty
• UniProt Gene Name: SPRY2
• UniProt Synonym Gene Names: Spry-2
• UniProt Entry Name: SPY2_HUMAN

NCBI Description

This gene encodes a protein belonging to the sprouty family. The encoded protein contains a carboxyl-terminal cysteine-rich domain essential for the inhibitory activity on receptor tyrosine kinase signaling proteins and is required for growth factor stimulated translocation of the protein to membrane ruffles. In primary dermal endothelial cells this gene is transiently upregulated in response to fibroblast growth factor two. This protein is indirectly involved in the non-cell autonomous inhibitory effect on fibroblast growth factor two signaling. The protein interacts with Cas-Br-M (murine) ectropic retroviral transforming sequence, and can function as a bimodal regulator of epidermal growth factor receptor/mitogen-activated protein kinase signaling. This protein may play a role in alveoli branching during lung development as shown by a similar mouse protein. [provided by RefSeq, Jul 2008]

Uniprot Description

SPRY2: a feedback inhibitor of growth factor receptor signaling. Belongs to the sprouty family. May function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis. Associated with microtubules in unstimulated cells but is translocated to the membrane ruffles in cells stimulated with EGF (epidermal growth factor).

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis; Inhibitor

Chromosomal Location of Human Ortholog: 13q31.1

Cellular Component: microtubule cytoskeleton; microtubule; plasma membrane; cytosol

Molecular Function: protein serine/threonine kinase inhibitor activity; protein serine/threonine kinase activator activity; protein binding; protein kinase binding

Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of epidermal growth factor receptor signaling pathway; negative regulation of MAP kinase activity; inner ear morphogenesis; cell fate commitment; fibroblast growth factor receptor signaling pathway; negative regulation of fibroblast growth factor receptor signaling pathway; negative regulation of nerve growth factor receptor signaling pathway; positive regulation of peptidyl-serine phosphorylation; negative regulation of cell proliferation; positive regulation of protein kinase B signaling cascade; sensory perception of sound; establishment of mitotic spindle orientation; negative regulation of Ras protein signal transduction; negative regulation of apoptosis