Rabbit anti-Human SPNS2 Polyclonal Antibody | anti-SPNS2 antibody

SPNS2, NT (SPNS2, Protein spinster homolog 2) (PE)

Cat. Num. AAA6350683

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SPNS2; Polyclonal Antibody; NT (SPNS2; Protein spinster homolog 2) (PE); Protein spinster homolog 2; anti-SPNS2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-SPNS2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SPNS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 67-94 amino acids from the N-terminal region of human SPNS2.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SPNS2 Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the SPNS2 antibody detected the SPNS2 protein (arrow).)

Related Product Information for anti-SPNS2 antibody

Sphingolipid transporter required for migration of myocardial precursors. Transports sphingosine 1-phosphate (S1P), a secreted lipid mediator that plays critical roles in cardiovascular, immunological, and neural development and function. Mediates the export of S1P from cells in the extraembryonic yolk syncytial layer (YSL), thereby regulating myocardial precursor migration.

Product Categories/Family for anti-SPNS2 antibody

Antibodies; Proteins

References

Kawahara, A., et al. Science 323(5913):524-527(2009). Yanagisawa, H., et al. Cell Death Differ. 10(7):798-807(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 185136021
• NCBI GeneID: 124976
• NCBI Accession #: NP_001118230.1
• NCBI GenBank Nucleotide #: NM_001124758.1
• UniProt Accession #: Q8IVW8; B9A1T3
• Molecular Weight: 58,044 Da
• NCBI Official Full Name: protein spinster homolog 2
• NCBI Official Synonym Full Names: spinster homolog 2 (Drosophila)
• NCBI Official Symbol: SPNS2
• NCBI Protein Information: protein spinster homolog 2
• UniProt Protein Name: Protein spinster homolog 2
• Protein Family: Protein spinster
• UniProt Gene Name: SPNS2
• UniProt Entry Name: SPNS2_HUMAN

Uniprot Description

SPNS2: Sphingolipid transporter required for migration of myocardial precursors. Transports sphingosine 1-phosphate (S1P), a secreted lipid mediator that plays critical roles in cardiovascular, immunological, and neural development and function. Mediates the export of S1P from cells in the extraembryonic yolk syncytial layer (YSL), thereby regulating myocardial precursor migration. Belongs to the major facilitator (TC 2.A.1) superfamily. Spinster (TC 2.A.1.49) family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17p13.2

Cellular Component: integral to membrane; lysosomal membrane; vesicle

Molecular Function: sphingolipid transporter activity

Biological Process: B cell homeostasis; lipid transport; locomotion; lymph node development; regulation of eye pigmentation; regulation of humoral immune response; sphingolipid metabolic process; T cell homeostasis; transmembrane transport