Human Neprilysin (NEP) ELISA Kit | NEP elisa kit

Human Neprilysin (NEP) ELISA Kit

Cat. Num. AAA451125

• Gene Names: MME; NEP; SFE; CD10; CALLA; CMT2T; SCA43
• Reactivity: Human
• Synonyms: Neprilysin (NEP); Human Neprilysin (NEP) ELISA Kit; CD10; MME; CALLA; SFE; Atriopeptidase; Enkephalinase; Skin fibroblast elastase; Common Acute Lymphoblastic Leukemia Antigen; Membrane Metallo-Endopeptidase; NEP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of NEP. No significant cross-reactivity or interference between NEP and analogues was observed.
• Sequence Length: 750

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 15.625-1000pg/mL
• Sensitivity: 5.8pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NEP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NEP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NEP elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NEP in human serum, plasma and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NEP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NEP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NEP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 116256329
• NCBI GeneID: 4311
• NCBI Accession #: NP_000893.2
• NCBI GenBank Nucleotide #: NM_000902.3
• UniProt Accession #: P08473; Q3MIX4; A8K6U6; D3DNJ9
• Molecular Weight: 85,514 Da
• NCBI Official Full Name: neprilysin
• NCBI Official Synonym Full Names: membrane metalloendopeptidase
• NCBI Official Symbol: MME
• NCBI Official Synonym Symbols: NEP; SFE; CD10; CALLA; CMT2T; SCA43
• NCBI Protein Information: neprilysin
• UniProt Protein Name: Neprilysin
• Protein Family: Halolysin-like extracellular serine protease
• UniProt Gene Name: MME
• UniProt Synonym Gene Names: EPN; CALLA; NEP; Neutral endopeptidase; SFE

NCBI Description

This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. This gene, which encodes a 100-kD type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5' untranslated region of this gene is alternatively spliced, resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing. [provided by RefSeq, Jul 2008]

Uniprot Description

Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids (PubMed:15283675, PubMed:8168535). Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond (PubMed:17101991). Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 (PubMed:15283675). Involved in the degradation of atrial natriuretic factor (ANF) (PubMed:2531377, PubMed:2972276). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed:20876573).