anti-Human, Rat AGFG1 Polyclonal Antibody | anti-AGFG1 antibody

Anti-AGFG1 Antibody

Cat. Num. AAA177887

• Gene Names: AGFG1; HRB; RAB; RIP
• Reactivity: Human, Rat
• Applications: Western Blot
• Purity: Immunogen Affinity Purified
• Synonyms: AGFG1; Polyclonal Antibody; Anti-AGFG1 Antibody; Arf-GAP domain and FG repeat-containing protein 1; AGFG1_HUMAN; Arf-GAP domain and FG repeats-containing protein 1; ArfGAP with FG repeats 1; AU045498; C130049H11Rik; C85612; D730048C23Rik; DKFZp686I15205; HIV 1 Rev binding protein; HIV-1 Rev-binding protein; HRB; MGC116375; MGC116938; MGC116940; Nucleoporin like protein RIP; Nucleoporin-like protein RIP; RAB; Rev interacting protein; Rev-interacting protein; Rev/Rex activation domain-binding protein; RIP; ; anti-AGFG1 antibody

• Reactivity: Human, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 584

• Applicable Applications for anti-AGFG1 antibody: Western Blot (WB)
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human AGFG1(5-28aa AKRKQEEKHLKMLRDMTGLPHNRK), identical to the related mouse and rat sequences.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- AGFG1 antibody, Western blottingAll lanes: Anti AGFG1 at 0.5ug/mlLane 1: Rat Testis Tissue Lysate at 50ugLane 2: Rat Liver Tissue Lysate at 50ugLane 3: Human Placenta Tissue Lysate at 50ugLane 4: MCF-7 Whole Cell Lysate at 40ugLane 5: HELA Whole Cell Lysate at 40ugPredicted bind size: 58KDObserved bind size: 58KD)

Related Product Information for anti-AGFG1 antibody

Description: Rabbit IgG polyclonal antibody for Arf-GAP domain and FG repeat-containing protein 1(AGFG1) detection. Tested with WB in Human;Rat.

Background: Arf-GAP domain and FG repeats-containing protein 1 is aprotein that in humans is encoded by the AGFG1 gene. AGFG1 is located to 2q36.3. The protein encoded by this gene is related to nucleoporins, a class of proteins that mediate nucleocytoplasmic transport. The encoded protein binds the activation domain of the human immunodeficiency virus Rev protein when Rev is assembled onto its RNA target, and is required for the nuclear export of Rev-directed RNAs. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

References

1. "Entrez Gene: HRB HIV-1 Rev binding protein". 2. Fritz CC, Zapp ML, Green MR (Sep 1995). "A human nucleoporin-like protein that specifically interacts with HIV Rev".Nature 376 (6540): 530-3.

NCBI and Uniprot Product Information

• NCBI GI #: 206597505
• NCBI GeneID: 3267
• NCBI Accession #: NP_001128659.1
• NCBI GenBank Nucleotide #: NM_001135187.1
• UniProt Accession #: P52594; Q15277; Q4VAS0; Q4VAS1; Q4VAS3; Q53QT8; Q53R11; B3KUL1; E9PHX7
• Molecular Weight: 60,767 Da
• NCBI Official Full Name: arf-GAP domain and FG repeat-containing protein 1 isoform 1
• NCBI Official Synonym Full Names: ArfGAP with FG repeats 1
• NCBI Official Symbol: AGFG1
• NCBI Official Synonym Symbols: HRB; RAB; RIP
• NCBI Protein Information: arf-GAP domain and FG repeat-containing protein 1
• UniProt Protein Name: Arf-GAP domain and FG repeat-containing protein 1
• Protein Family: Arf-GAP domain and FG repeat-containing protein
• UniProt Gene Name: AGFG1
• UniProt Synonym Gene Names: HRB; RAB; RIP
• UniProt Entry Name: AGFG1_HUMAN

NCBI Description

The protein encoded by this gene is related to nucleoporins, a class of proteins that mediate nucleocytoplasmic transport. The encoded protein binds the activation domain of the human immunodeficiency virus Rev protein when Rev is assembled onto its RNA target, and is required for the nuclear export of Rev-directed RNAs. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

RIP: Required for vesicle docking or fusion during acrosome biogenesis. May play a role in RNA trafficking or localization. In case of infection by HIV-1, acts as a cofactor for viral Rev and promotes movement of Rev-responsive element- containing RNAs from the nuclear periphery to the cytoplasm. This step is essential for HIV-1 replication. Interacts with EPS15R and EPS15. Interacts with FCHO1. Ubiquitously expressed. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Nuclear export

Chromosomal Location of Human Ortholog: 2q36.3

Cellular Component: cell projection; cell soma; cytoplasmic membrane-bound vesicle; intracellular membrane-bound organelle; nuclear pore

Molecular Function: DNA binding; GTPase activator activity; metal ion binding; protein binding; RNA binding

Biological Process: acrosome formation; intermediate filament organization; mRNA export from nucleus; multicellular organismal development; positive regulation of GTPase activity; spermatid nuclear differentiation