Rabbit SPIB Polyclonal Antibody | anti-SPIB antibody

Anti-SPIB Antibody, Rabbit Polyclonal

Cat. Num. AAA8101279

• Gene Names: SPIB; SPI-B
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Applications: Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: SPIB; Polyclonal Antibody; Anti-SPIB Antibody; Rabbit Polyclonal; SPIB Antibody; Rabbit PAb; Antigen Affinity Purified; Spi-B transcription factor (Spi-1/PU.1 related); Anti-SPI-B Antibody; anti-SPIB antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human SPIB
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-SPIB antibody: Immunoprecipitation (IP)
• Application Notes: IP: 4-8uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human SPIB
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human SPIB, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(SPIB was immunoprecipitated using: Lane A:0.5 mg NIH3T3 Whole Cell Lysate 4 uL anti-SPIB rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-SPIB rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 42 kDa Observed band size: 44 kDa)

Related Product Information for anti-SPIB antibody

SPIB, an Ets family transcription factor linked to lymphomagenesis, in DLBCL. SPIB expression is a clinically novel poor prognostic factor in DLBCL that contributes to treatment resistance, at least in part, through an anti-apoptotic mechanism. SPIB is an Ets transcription factor that is expressed exclusively in mature B cells, T-cell progenitors, and plasmacytoid dendritic cells. SPIB expression can be used as a tool for testing BPDC neoplasms, but it needs to be tested in conjunction with the growing arsenal of markers for human plasmacytoid dendritic cells.

NCBI and Uniprot Product Information

• NCBI GI #: 61888836
• NCBI GeneID: 6689
• NCBI Accession #: NP_003112
• NCBI GenBank Nucleotide #: NM_003121.4
• UniProt Accession #: Q01892; Q15359; A8K9C9; B4DUG6
• Molecular Weight: Predicted: 19; 25; 29 kDa; ; Observed: 20 kDa
• NCBI Official Full Name: transcription factor Spi-B isoform 1
• NCBI Official Synonym Full Names: Spi-B transcription factor (Spi-1/PU.1 related)
• NCBI Official Symbol: SPIB
• NCBI Official Synonym Symbols: SPI-B
• NCBI Protein Information: transcription factor Spi-B
• UniProt Protein Name: Transcription factor Spi-B
• Protein Family: Transcription factor
• UniProt Gene Name: SPIB
• UniProt Entry Name: SPIB_HUMAN

NCBI Description

The protein encoded by this gene is a transcriptional activator that binds to the PU-box (5'-GAGGAA-3') and acts as a lymphoid-specific enhancer. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

SPIB: Sequence specific transcriptional activator which binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Promotes development of plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells. These cells have the capacity to produce large amounts of interferon and block viral replication. May be required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation. Belongs to the ETS family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: cytoplasm; nucleus

Molecular Function: sequence-specific DNA binding

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; cell differentiation