Western Blot (WB)
(SLC36A1 Antibody (N-term) (MBS9211275) western blot analysis in NCI-H460 (lane 1), K562 (lane 2), A549 (lane 3) cell line lysates (35ug/lane). This demonstrates the SLC36A1 antibody detected the SLC36A1 protein (arrow).)
Rabbit SLC36A1 Polyclonal Antibody | anti-SLC36A1 antibody
SLC36A1 Antibody (N-term)
WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Western Blot (WB)
(SLC36A1 Antibody (N-term) (MBS9211275) western blot analysis in NCI-H460 (lane 1), K562 (lane 2), A549 (lane 3) cell line lysates (35ug/lane). This demonstrates the SLC36A1 antibody detected the SLC36A1 protein (arrow).)
Western Blot (WB)
(SLC36A1 Antibody (N-term) (MBS9211275) western blot analysis in NCI-H460 (lane 1), K562 (lane 2), A549 (lane 3) cell line lysates (35ug/lane). This demonstrates the SLC36A1 antibody detected the SLC36A1 protein (arrow).)
Immunohistochemistry (IHC)
(SLC36A1 Antibody (N-term) (MBS9211275) immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of SLC36A1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(SLC36A1 Antibody (N-term) (MBS9211275) immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of SLC36A1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of SLC36A1 Antibody (N-term) (MBS9211275) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of SLC36A1 Antibody (N-term) (MBS9211275) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
Flow Cytometry (FC/FACS)
(SLC36A1 Antibody (N-term) (MBS9211275) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(SLC36A1 Antibody (N-term) (MBS9211275) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Dorn, M., et al. FEBS Lett. 583(10):1631-1636(2009)
Metzner, L., et al. Mol. Pharm. 6(3):1006-1011(2009)
Anderson, C.M., et al. J. Physiol. (Lond.) 587 (PT 4), 731-744 (2009) :
Metzner, L., et al. Biochim. Biophys. Acta 1778(4):1042-1050(2008)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the eukaryote-specific amino acid/auxin permease (AAAP) 1 transporter family. The encoded protein functions as a proton-dependent, small amino acid transporter. This gene is clustered with related family members on chromosome 5q33.1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]
Uniprot Description
SLC36A1: Neutral amino acid/proton symporter. Has a pH-dependent electrogenic transport activity for small amino acids such as glycine, alanine and proline. Besides small apolar L-amino acids, it also recognize their D-enantiomers and selected amino acid derivatives such as gamma-aminobutyric acid. Belongs to the amino acid/polyamine transporter 2 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Transporter, SLC family; Membrane protein, multi-pass; Transporter
Chromosomal Location of Human Ortholog: 5q33.1
Cellular Component: lysosomal membrane; endoplasmic reticulum; plasma membrane; integral to membrane
Molecular Function: glycine transmembrane transporter activity; L-proline transmembrane transporter activity; hydrogen:amino acid symporter activity; hydrogen ion transmembrane transporter activity; L-alanine transmembrane transporter activity
Biological Process: glycine transport; proton transport; amino acid transport; ion transport; transmembrane transport; L-alanine transport
Research Articles on SLC36A1
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Product Notes
The SLC36A1 slc36a1 (Catalog #AAA9211275) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-30. The SLC36A1 Antibody (N-term) reacts with Human (Predicted Reactivity: Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's SLC36A1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Flow Cytometry (FC/FACS). IF~~1:10~50 WB~~1:1000 IHC-P~~1:10~50 FC~~1:10~50. Researchers should empirically determine the suitability of the SLC36A1 slc36a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SLC36A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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