Western Blot (WB)
(Anti-TSC22D1 rabbit polyclonal antibody at 1:500 dilution Lane A: MCF7 Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:110 kDa Observed band size:120 kDa (We are unsure as to the identity of these extra bands.))
Rabbit TSC22D1 Polyclonal Antibody | anti-TSC22D1 antibody
Anti-TSC22D1 Antibody, Rabbit Polyclonal
Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
IP: 1-4uL/mg of lysate
Western Blot (WB)
(Anti-TSC22D1 rabbit polyclonal antibody at 1:500 dilution Lane A: MCF7 Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:110 kDa Observed band size:120 kDa (We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-TSC22D1 rabbit polyclonal antibody at 1:500 dilution Lane A: MCF7 Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:110 kDa Observed band size:120 kDa (We are unsure as to the identity of these extra bands.))
Immunoprecipitation (IP)
(TSC22D1 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate Lane B:0.5 mg Jurkat Whole Cell Lysate 4 uL anti-TSC22D1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-TSC22D1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 110 kDa Observed band size: 110 kDa)
Immunoprecipitation (IP)
(TSC22D1 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate Lane B:0.5 mg Jurkat Whole Cell Lysate 4 uL anti-TSC22D1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-TSC22D1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 110 kDa Observed band size: 110 kDa)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the TSC22 domain family of leucine zipper transcription factors. The encoded protein is stimulated by transforming growth factor beta, and regulates the transcription of multiple genes including C-type natriuretic peptide. The encoded protein may play a critical role in tumor suppression through the induction of cancer cell apoptosis, and a single nucleotide polymorphism in the promoter of this gene has been associated with diabetic nephropathy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]
Uniprot Description
TSC22D1: Transcriptional repressor. Acts on the C-type natriuretic peptide (CNP) promoter. Belongs to the TSC-22/Dip/Bun family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 13q14
Cellular Component: cytoplasm; nucleus
Molecular Function: protein binding; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; positive regulation of apoptosis; positive regulation of cell proliferation; negative regulation of apoptosis
Research Articles on TSC22D1
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Product Notes
The TSC22D1 tsc22d1 (Catalog #AAA8101325) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TSC22D1 Antibody, Rabbit Polyclonal reacts with Human Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. AAA Biotech's TSC22D1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:2000 IP: 1-4uL/mg of lysate. Researchers should empirically determine the suitability of the TSC22D1 tsc22d1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TSC22D1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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