Western Blot (WB)
(Western Blot: Sample: Human Hela cell lysate; Primary Ab: 3ug/ml Rabbit Anti-Human GP4 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Human Platelet Membrane Glycoprotein IV (GP4) Polyclonal Antibody | anti-GP4 antibody
Polyclonal Antibody to Platelet Membrane Glycoprotein IV (GP4)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LSLV PYPVTTTVGL FYPYNNTADG VYKVFNGKDN ISKVAIIDTY KGKRNLSYWE SHCDMINGTD AASFPPFVEK SQVLQFFSSD ICRSIYAVFE SDVNLKGIPV YRFVLPSKAF ASPVENPDNY CFCTEKIISK NCTSYGVLDI SKCKEGRPVY ISLPHFLYAS PDVSEPIDGL NPNEEEHRTY LDIEPITGFT
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Human Hela cell lysate; Primary Ab: 3ug/ml Rabbit Anti-Human GP4 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Human Hela cell lysate; Primary Ab: 3ug/ml Rabbit Anti-Human GP4 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Stomach tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Stomach tissue))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type HepG2 cell lysate; Lane 2: GP4 knockout HepG2 cell lysate; Predicted MW: 53kd Observed MW: 68kd Primary Ab: 3ug/ml Rabbit Anti-Human GP4 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type HepG2 cell lysate; Lane 2: GP4 knockout HepG2 cell lysate; Predicted MW: 53kd Observed MW: 68kd Primary Ab: 3ug/ml Rabbit Anti-Human GP4 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is the fourth major glycoprotein of the platelet surface and serves as a receptor for thrombospondin in platelets and various cell lines. Since thrombospondins are widely distributed proteins involved in a variety of adhesive processes, this protein may have important functions as a cell adhesion molecule. It binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. It directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and it binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2014]
Uniprot Description
CD36: Seems to have numerous potential physiological functions. Binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. May function as a cell adhesion molecule. Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Receptor for thombospondins, THBS1 AND THBS2, mediating their antiangiogenic effects. Defects in CD36 are the cause of platelet glycoprotein IV deficiency (PG4D)[MIM:608404]; also known as CD36 deficiency. Platelet glycoprotein IV deficiency can be divided into 2 subgroups. The type I phenotype is characterized by platelets and monocytes/macrophages exhibiting complete CD36 deficiency. The type II phenotype lacks the surface expression of CD36 in platelets, but expression in monocytes/macrophages is near normal. Genetic variations in CD36 are associated with susceptibility to coronary heart disease type 7 (CHDS7). Belongs to the CD36 family.
Protein type: Cell adhesion; Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 7q21.11
Cellular Component: apical plasma membrane; brush border membrane; cell surface; external side of plasma membrane; extracellular space; Golgi apparatus; integral component of plasma membrane; intracellular; membrane; membrane raft; phagocytic vesicle; plasma membrane; platelet alpha granule membrane; receptor complex
Molecular Function: amyloid-beta binding; high-density lipoprotein particle binding; lipid binding; low-density lipoprotein particle binding; low-density lipoprotein receptor activity; protein binding; scavenger receptor activity; transforming growth factor beta binding
Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; apoptotic cell clearance; blood coagulation; cell adhesion; cell surface receptor signaling pathway; cGMP-mediated signaling; cholesterol transport; defense response to Gram-positive bacterium; elevation of cytosolic calcium ion concentration; interleukin-1 beta secretion; intestinal absorption; intestinal cholesterol absorption; lipid metabolic process; lipid storage; lipoprotein transport; low density lipoprotein mediated signaling; MyD88-dependent toll-like receptor signaling pathway; negative regulation of transcription factor import into nucleus; negative regulation of transcription from RNA polymerase II promoter; neutrophil degranulation; nitric oxide mediated signal transduction; phagocytosis, recognition; platelet degranulation; positive regulation of blood coagulation; positive regulation of cell-matrix adhesion; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of interleukin-12 production; positive regulation of interleukin-6 production; positive regulation of NF-kappaB transcription factor activity; positive regulation of nitric oxide biosynthetic process; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phagocytosis, engulfment; positive regulation of tumor necrosis factor production; receptor internalization; receptor-mediated endocytosis; regulation of lipid metabolic process; regulation of protein heterodimerization activity; regulation of toll-like receptor signaling pathway; response to lipid; sensory perception of taste; toll-like receptor signaling pathway; triglyceride transport
Disease: Coronary Heart Disease, Susceptibility To, 7; Malaria, Susceptibility To; Platelet Glycoprotein Iv Deficiency
Research Articles on GP4
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Product Notes
The GP4 cd36 (Catalog #AAA2015776) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Platelet Membrane Glycoprotein IV (GP4) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Platelet Membrane Glycoprotein IV (GP4) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the GP4 cd36 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LSLV PYPVTTTVGL FYPYNNTADG VYKVFNGKDN ISKVAIIDTY KGKRNLSYWE SHCDMINGTD AASFPPFVEK SQVLQFFSSD ICRSIYAVFE SDVNLKGIPV YRFVLPSKAF ASPVENPDNY CFCTEKIISK NCTSYGVLDI SKCKEGRPVY ISLPHFLYAS PDVSEPIDGL NPNEEEHRTY LDIEPITGFT. It is sometimes possible for the material contained within the vial of "Platelet Membrane Glycoprotein IV (GP4), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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