Typical Testing Data/Standard Curve (for reference only)
Mouse Bifunctional purine biosynthesis protein PURH [Includes: Phosphoribosylaminoimidazolecarboxamide formyltransferase (ATIC) ELISA Kit | ATIC elisa kit
Mouse Bifunctional purine biosynthesis protein PURH [Includes: Phosphoribosylaminoimidazolecarboxamide formyltransferase (ATIC) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: ATHL1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ATHL1 antibody and an ATHL1-HRP conjugate. The assay sample and buffer are incubated together with ATHL1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATHL1 concentration since ATHL1 from samples and ATHL1-HRP conjugate compete for the anti-ATHL1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATHL1 from the sample, fewer sites are left to bind ATHL1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATHL1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a bifunctional protein that catalyzes the last two steps of the de novo purine biosynthetic pathway. The N-terminal domain has phosphoribosylaminoimidazolecarboxamide formyltransferase activity, and the C-terminal domain has IMP cyclohydrolase activity. A mutation in this gene results in AICA-ribosiduria. [provided by RefSeq, Sep 2009]
Uniprot Description
ATIC: Bifunctional enzyme that catalyzes 2 steps in purine biosynthesis. Defects in ATIC are the cause of AICAR transformylase/IMP cyclohydrolase deficiency (AICAR). A neurologically devastating inborn error of purine biosynthesis. Patients excrete massive amounts of AICA-riboside in the urine and accumulate AICA- ribotide and its derivatives in erythrocytes and fibroblasts. AICAR causes profound mental retardation, epilepsy, dysmorphic features and congenital blindness. Belongs to the PurH family.
Protein type: Methyltransferase; Hydrolase; Nucleotide Metabolism - purine; Cofactor and Vitamin Metabolism - one carbon pool by folate; EC 3.5.4.10; EC 2.1.2.3
Chromosomal Location of Human Ortholog: 2q35
Cellular Component: membrane; mitochondrion; cytosol
Molecular Function: protein homodimerization activity; IMP cyclohydrolase activity; phosphoribosylaminoimidazolecarboxamide formyltransferase activity
Biological Process: purine ribonucleoside monophosphate biosynthetic process; organ regeneration; tetrahydrofolate biosynthetic process; response to inorganic substance; nucleobase, nucleoside and nucleotide metabolic process; dihydrofolate metabolic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; nucleoside metabolic process; cerebellum development; cerebral cortex development; purine base metabolic process; 'de novo' IMP biosynthetic process
Disease: Aicar Transformylase/imp Cyclohydrolase Deficiency
Research Articles on ATIC
Similar Products
Product Notes
The Mouse ATIC atic (Catalog #AAA7220074) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7220074 ELISA Kit recognizes Mouse ATIC. It is sometimes possible for the material contained within the vial of "Bifunctional purine biosynthesis protein PURH [Includes: Phosphoribosylaminoimidazolecarboxamide formyltransferase (ATIC), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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