Rabbit anti-Human, Mouse SPECC1 Polyclonal Antibody | anti-SPECC1 antibody

SPECC1, ID (SPECC1L, CYTSA, KIAA0376, Cytospin-A, Renal carcinoma antigen NY-REN-22, Sperm antigen with calponin homology and coiled-coil domains 1-like) (MaxLight 550)

Cat. Num. AAA6350471

• Gene Names: SPECC1L; TBHS; CYTSA; GBBB2; OBLFC1
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SPECC1; Polyclonal Antibody; ID (SPECC1L; CYTSA; KIAA0376; Cytospin-A; Renal carcinoma antigen NY-REN-22; Sperm antigen with calponin homology and coiled-coil domains 1-like) (MaxLight 550); SPECC1L; Sperm antigen with calponin homology and coiled-coil domains 1-like; anti-SPECC1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SPECC1. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.
• Sequence Length: 1117

• Applicable Applications for anti-SPECC1 antibody: Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IF: 1:10-50; IHC: 1:10-50; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 705-733 in the central region of human SPECC1, UniProt Accession #Q69YQ0. Species sequence homology: chicken, zebrafish
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in HL-60 cell line lysates (35ug/lane) usingMBS6001893. This demonstrates that MBS6001893 detected the SPECC1 protein (arrow).)

Western Blot (WB)

(Western Blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane) usingMBS6001893. This demonstrates that MBS6001893 detected the SPECC1 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue using MBS6001893 followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SPECC1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow Cytometry analysis of HL-60 cells using MBS6001893 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of U-251MG cells using MBS6001893 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-SPECC1 antibody

CYTSAis involved in cytokinesis and spindle organization.

Product Categories/Family for anti-SPECC1 antibody

Antibodies; Cancer Markers

References

1. Scanlan, M.J., et al. Int. J. Cancer 83(4):456-464(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 224451082
• NCBI GeneID: 23384
• NCBI Accession #: NP_001138940.1
• NCBI GenBank Nucleotide #: NP_001138940.1
• UniProt Accession #: Q69YQ0
• NCBI Official Full Name: cytospin-A
• NCBI Official Synonym Full Names: sperm antigen with calponin homology and coiled-coil domains 1 like
• NCBI Official Symbol: SPECC1L
• NCBI Official Synonym Symbols: TBHS; CYTSA; GBBB2; OBLFC1
• NCBI Protein Information: cytospin-A
• UniProt Protein Name: Cytospin-A
• Protein Family: Cytospin
• UniProt Gene Name: SPECC1L
• UniProt Synonym Gene Names: CYTSA; KIAA0376; SPECC1-like protein
• UniProt Entry Name: CYTSA_HUMAN

NCBI Description

This gene encodes a coiled-coil domain containing protein. The encoded protein may play a critical role in actin-cytoskeletal reorganization during facial morphogenesis. Mutations in this gene are a cause of oblique facial clefting-1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. A read-through transcript composed of SPECC1L (sperm antigen with calponin homology and coiled-coil domains 1-like) and the downstream ADORA2A (adenosine A2a receptor) gene sequence has been identified, but it is thought to be non-coding. [provided by RefSeq, Jun 2013]

Uniprot Description

Function: Involved in cytokinesis and spindle organization. May play a role in actin cytoskeleton organization and microtubule stabilization and hence required for proper cell adhesion and migration. Ref.10

Subunit structure: May interact with both microtubules and actin cytoskeleton.

Subcellular location: Cytoplasm › cytoskeleton. Cytoplasm › cytoskeleton › spindle. Cell junction › gap junction. Note: Colocalizes with acetylated alpha-tubulin, gamma-tubulin and F-actin. Also observed in a ring around gamma-tubulin containing centrioles possibly in the microtubule organizing center. Ref.10

Involvement in disease: Facial clefting, oblique, 1 (OBLFC1) [MIM:600251]: A rare form of facial clefting. A facial cleft is any of the fissures between the embryonic prominences that normally unite to form the face.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.10

Sequence similarities: Belongs to the cytospin-A family.Contains 1 CH (calponin-homology) domain.