Rabbit anti-Human SPANXC / SPANX-C Polyclonal Antibody | anti-SPANXC antibody

Rabbit Polyclonal to Human SPANXC / SPANX-C

Cat. Num. AAA240445

• Gene Names: SPANXC; C; CTp11; CT11.3; SPANX-C
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: SPANXC / SPANX-C; Polyclonal Antibody; Rabbit Polyclonal to Human SPANXC / SPANX-C; Anti-SPANXC / SPANX-C Antibody IHC-plus; SPANXC; CTp11; CT11.3; SPANX family member C; SPAN-Xc protein; SPANX family; member C; SPANX-C; Cancer/testis antigen 11.3; Human SPANXC; anti-SPANXC antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Full-length recombinant human SPANX-C protein.
• Purity/Purification: Protein A Purified
• Form/Format: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 97

• Applicable Applications for anti-SPANXC antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:5000 - 1:20000), IHC-P (2.5 ug/ml), WB (1:1000 - 1:5000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Immunogen Description: Recombinant human SPANXC.
• Immunogen Type: Recombinant protein
• Immunogen: SPANXC / SPANX-C antibody was raised against recombinant human SPANXC.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-SPANXC antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Anti-SPANX-C Antibody - Western Blot. Western blot of Protein A purified anti-SPANX-C antibody shows detection of a band at ~17 kD corresponding to SPANX-C present in a nuclear extract from VWM105 cells (left panel, arrowhead). VWM105 cells are derived from a human melanoma and are positive for SPANX proteins. Lane 2 shows reactivity with a purified recombinant SPANX-C fusion protein. The right panel shows similar reactivity with purified recombinant SPANX-B, SPANX-C and SPANX-N proteins. Proteins were separated by SDS-PAGE, transferred to nitrocellulose, and probed with the primary antibody diluted to 1:1000. IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (was used (left). IRDye is a trademark of LI-COR, Inc. Size estimation was made by comparison to prestained MW markers as indicated. Personal Communication. Vladimir Larionov, NIH, CCR, Bethesda, MD.)

Related Product Information for anti-SPANXC antibody

Sperm Protein Associated with the Nucleus, X Chromosome, family member C (SPANX-C) was originally isolated in a search for genes that contribute to the metastatic pathway of neoplastic cells. Expression in melanoma cells was found in only the most aggressive, highly metastatic cell lines. Subsequent studies have shown expression in a low percentage of carcinomas and in testis.

NCBI and Uniprot Product Information

• NCBI GI #: 13435137
• NCBI GeneID: 64663
• NCBI Accession #: NP_073152.1
• NCBI GenBank Nucleotide #: NM_022661.2
• UniProt Accession #: Q9NY87; Q32WL9; Q5JX88
• Molecular Weight: 11,002 Da
• NCBI Official Full Name: sperm protein associated with the nucleus on the X chromosome C
• NCBI Official Synonym Full Names: SPANX family, member C
• NCBI Official Symbol: SPANXC
• NCBI Official Synonym Symbols: C; CTp11; CT11.3; SPANX-C
• NCBI Protein Information: sperm protein associated with the nucleus on the X chromosome C; SPAN-Xc protein; cancer/testis antigen 11.3; nuclear-associated protein SPAN-Xc; cancer-testis-associated protein CTp11; cancer/testis-associated protein CTp11; cancer/testis antigen family 11, member 3; cancer/testis-associated protein of 11 kD; sperm protein associated with the nucleus, X chromosome, family member C
• UniProt Protein Name: Sperm protein associated with the nucleus on the X chromosome C
• Protein Family: Sperm protein associated with the nucleus on the X chromosome
• UniProt Gene Name: SPANXC
• UniProt Synonym Gene Names: CT11.3; SPANX-C
• UniProt Entry Name: SPNXC_HUMAN

NCBI Description

Temporally regulated transcription and translation of several testis-specific genes is required to initiate the series of molecular and morphological changes in the male germ cell lineage necessary for the formation of mature spermatozoa. This gene is a member of the SPANX family, which is located in a gene cluster on chromosome X. The SPANX genes encode differentially expressed testis-specific proteins that localize to various subcellular compartments. This particular gene encodes a protein that localizes to the nucleus and is expressed in highly metastatic cell lines, making the protein a potential diagnostic and prognostic marker. The protein belongs to a family of cancer/testis antigens and represents a potential target for cancer immunotherapy. [provided by RefSeq, Jul 2008]

Uniprot Description

SPANXC: Temporally regulated transcription and translation of several testis-specific genes is required to initiate the series of molecular and morphological changes in the male germ cell lineage necessary for the formation of mature spermatozoa. This gene is a member of the SPANX family, which is located in a gene cluster on chromosome X. The SPANX genes encode differentially expressed testis-specific proteins that localize to various subcellular compartments. This particular gene encodes a protein that localizes to the nucleus and is expressed in highly metastatic cell lines, making the protein a potential diagnostic and prognostic marker. The protein belongs to a family of cancer/testis antigens and represents a potential target for cancer immunotherapy. [provided by RefSeq, Jul 2008]

Protein type: Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: Xq27.1

Cellular Component: cytoplasm; nucleus