<
Western Blot (WB)
(Western Blot analysis of SOX2 recombinant protein using 031255. SOX2(arrow) was detected using the 031255.)
Mouse anti-Human SOX2 Monoclonal Antibody | anti-SOX2 antibody
SOX2 (SOX2, Transcription factor SOX-2) (MaxLight 550)
Gene Names
SOX2; ANOP3; MCOPS3
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified by Protein G Affinity Chromatography
Synonyms
SOX2; Monoclonal Antibody; SOX2 (SOX2; Transcription factor SOX-2) (MaxLight 550); Transcription factor SOX-2; anti-SOX2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
57CT23.3.4
Specificity
Recognizes human SOX2.
Purity/Purification
Purified by Protein G Affinity Chromatography
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.
Applicable Applications for anti-SOX2 antibody
Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Recombinant protein corresponding to human SOX2.
Conjugate
MaxLight550
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of SOX2 recombinant protein using 031255. SOX2(arrow) was detected using the 031255.)
Western Blot (WB)
(Western Blot analysis of SOX2 recombinant protein using 031255. SOX2(arrow) was detected using the 031255.)
Western Blot (WB)
(Western Blot analysis of SOX2 (arrow) 293 cell lysates (2ug/lane) using 031255, either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (Lane 2) (Origene Technologies))
Western Blot (WB)
(Western Blot analysis of SOX2 (arrow) 293 cell lysates (2ug/lane) using 031255, either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (Lane 2) (Origene Technologies))
Immunohistochemistry (IHC)
(Immunohistochemistry of formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with 031255, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry.)
Immunohistochemistry (IHC)
(Immunohistochemistry of formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with 031255, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry.)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of NCI-H460 cells using 031255 (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysi.)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of NCI-H460 cells using 031255 (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysi.)
Immunofluorescence (IF)
(Fluorescent image of A549 cell stained with 031255. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SOX2 primary antibody (1:25, 1 h at 37ºC). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37ºC). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37ºC). SOX2 immunoreactivity is localized to Nucleus significantly.)
Immunofluorescence (IF)
(Fluorescent image of A549 cell stained with 031255. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SOX2 primary antibody (1:25, 1 h at 37ºC). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37ºC). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37ºC). SOX2 immunoreactivity is localized to Nucleus significantly.)
Product Categories/Family for anti-SOX2 antibody
References
1. Zhang D, et al. BMC Med Genet, 2010 Jul 28. PMID 20667095. 2. Sholl LM, et al. Am J Surg Pathol, 2010 Aug. PMID 20631605. 3. Ge Y, et al. Biochem Biophys Res Commun, 2010 Jul 9. PMID 20537983. 4. Gonzalez-Rodriguez J, et al. Br J Ophthalmol, 2010 Aug. PMID 20494911. 4. Reis LM, et al. Mol Vis, 2010 Apr 28. PMID 20454695. References for SY5Y (SH-SY5Y; ATCC#CRL-2266): 1. Ross RA, et al. J. Natl. Cancer Inst. 71: 741-749, 1983. [PubMed: 6137586]; 2. Biedler JL, et al. Cancer Res. 38: 3751-3757, 1978. [PubMed: 29704]. References for MCF7 cell line:1. Soule, HD; et al, (1973). Journal of the National Cancer Institute 51 (5): 1409-1416. [PMID 4357757]. 2 Levenson, AS; Jordan VC. (1997). Cancer Research 57 (15): 3071-3078. [PMID 9242427]. 3. Lacroix, M; Leclercq G. (2004). Breast Research and Treatment 83(3): 249-289.[PMID 14758095].
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34kDa
NCBI Official Full Name
transcription factor SOX-2
NCBI Official Synonym Full Names
SRY (sex determining region Y)-box 2
NCBI Official Symbol
SOX2
NCBI Official Synonym Symbols
ANOP3; MCOPS3
NCBI Protein Information
transcription factor SOX-2; transcription factor SOX2; SRY-related HMG-box gene 2
UniProt Protein Name
Transcription factor SOX-2
UniProt Gene Name
SOX2
UniProt Entry Name
SOX2_HUMAN
Similar Products
Product Notes
The SOX2 sox2 (Catalog #AAA6209988) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SOX2 (SOX2, Transcription factor SOX-2) (MaxLight 550) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SOX2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the SOX2 sox2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SOX2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
