Rabbit Sp2 Polyclonal Antibody | anti-Sp2 antibody

Sp2 (D512) Polyclonal Antibody

Cat. Num. AAA3002072

• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Sp2; Polyclonal Antibody; Sp2 (D512) Polyclonal Antibody; Transcription factor Sp2; KIAA0048; SP2; anti-Sp2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Sp2 (D512) polyclonal antibody detects endogenous levels of Sp2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 613

• Applicable Applications for anti-Sp2 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 500-550 of Human Sp2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Testing Data

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Sp2 (D512) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Testing Data

Related Product Information for anti-Sp2 antibody

The Sp transcription factor family includes Sp1, Sp2, Sp3 (SPR-2) and Sp4(SPR-1). Sp transcription factors share similar structures but do not share similar functions. All four proteins contain a highly conserved DNA-binding domain composed of three zinc fingers at the C-terminus. Sp family members bind the consensus sequence GGGGCGGGGC and other closely related sequences which are known as GC boxes. Sp1, Sp3 and Sp4 share a high affinity for GC boxes while Sp2 does not. Sp2 only weakly binds to GT boxes. Sp1, Sp2 and Sp3 are ubiquitously expressed, while Sp4 is abundantly expressed in brain with limited expression in other tissues. Sp1 and Sp3, but not Sp2 or Sp4, interact with E2, a regulatory element for the b4 subunit of neuronal nicotinic acetylcholine receptors. Sp3 is the only Sp member to inhibit Sp1 and Sp4 mediated transcription. The gene3 encoding human Sp2 maps to chromosome 17p32.3.

NCBI and Uniprot Product Information

• NCBI GI #: 125625357
• NCBI Accession #: NP_003101.3
• NCBI GenBank Nucleotide #: NP_003101.3
• UniProt Accession #: Q02086
• Molecular Weight: ~ 64kDa
• NCBI Official Full Name: transcription factor Sp2
• UniProt Protein Name: Transcription factor Sp2
• Protein Family: Sex-specific storage-protein
• UniProt Gene Name: SP2
• UniProt Synonym Gene Names: KIAA0048
• UniProt Entry Name: SP2_HUMAN

Uniprot Description

SP2: Binds to GC box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites. Belongs to the Sp1 C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: nucleoplasm; nucleus

Molecular Function: DNA binding; histone deacetylase binding; metal ion binding

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; fibroblast proliferation; in utero embryonic development; multicellular organism growth; embryonic organ development; immune response