Rat Lactase (LCT) ELISA Kit | LCT elisa kit

Rat Lactase (LCT) ELISA Kit

Cat. Num. AAA2000039

• Gene Names: LCT; LAC; LPH; LPH1
• Reactivity: Rat
• Synonyms: Lactase (LCT); Rat Lactase (LCT) ELISA Kit; LAC; LPH; LPH1; Lactose-Phlorizin Hydrolase; LCT elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Lactase (LCT).; No significant cross-reactivity or interference between Lactase (LCT) and analogues was observed.
• Sequence Length: 1927

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.116ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lactase (LCT) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lactase (LCT) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LCT elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lactase (LCT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lactase (LCT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lactase (LCT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lactase (LCT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for LCT elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 119632027
• NCBI GeneID: 3938
• UniProt Accession #: P09848; Q4ZG58
• Molecular Weight: 218,587 Da
• NCBI Official Full Name: lactase
• NCBI Official Synonym Full Names: lactase
• NCBI Official Symbol: LCT
• NCBI Official Synonym Symbols: LAC; LPH; LPH1
• NCBI Protein Information: lactase-phlorizin hydrolase
• UniProt Protein Name: Lactase-phlorizin hydrolase
• Protein Family: Lactase-phlorizin hydrolase
• UniProt Gene Name: LCT
• UniProt Synonym Gene Names: LPH
• UniProt Entry Name: LPH_HUMAN

NCBI Description

The protein encoded by this gene belongs to the family 1 of glycosyl hydrolases. The protein is integral to plasma membrane and has both phlorizin hydrolase activity and lactase activity. [provided by RefSeq, Jul 2008]

Uniprot Description

LCT: LPH splits lactose in the small intestine. Defects in LCT are the cause of congenital lactase deficiency (COLACD); also known as hereditary alactasia or disaccharide intolerance II. Congenital lactase deficiency is a an autosomal recessive, rare and severe gastrointestinal disorder. It is characterized by watery diarrhea in infants fed with breast milk or other lactose-containing formulas. An almost total lack of LCT activity is found in jejunal biopsy material of patients with congenital lactase deficiency. Opposite to congenital lactase deficiency, adult-type hypolactasia, also known as lactose intolerance, is the most common enzyme deficiency worldwide. It is caused by developmental down-regulation of lactase activity during childhood or early adulthood. The decline of lactase activity is a normal physiological phenomenon; however, the majority of Northern Europeans have the ability to maintain lactase activity and digest lactose throughout life (lactase persistence). The down-regulation of lactase activity operates at the transcriptional level and it is associated with a noncoding variation in the MCM6 gene, located in the upstream vicinity of LCT. Belongs to the glycosyl hydrolase 1 family.

Protein type: EC 3.2.1.62; Membrane protein, integral; Carbohydrate Metabolism - galactose; Hydrolase; EC 3.2.1.108

Chromosomal Location of Human Ortholog: 2q21

Cellular Component: membrane; integral to plasma membrane; apical plasma membrane; plasma membrane

Molecular Function: lactase activity; glycosylceramidase activity

Biological Process: polysaccharide digestion; carbohydrate metabolic process; pathogenesis

Disease: Lactase Deficiency, Congenital