Testing Data
(EC=1.1.3.4; GOx; Notatin; Glucose oxyhydrase, Beta-D-glucose, Oxygen 1-oxidoreductaase)
Glucose Oxidase, A. niger Enzyme | gox enzyme
Glucose Oxidase, A. niger
Purity
Molecular Biology GradeChromatographically purified
Synonyms
Glucose Oxidase; A. niger; gox enzyme
Host
Aspergillus niger
Specificity
The enzyme is highly specific for beta-D-glucose. The alpha anomer is not acted upon; 2-deoxy-D-glucose, D-mannose and D-galactose exhibit low activities as substrate.
Purity/Purification
Molecular Biology Grade
Chromatographically purified
Chromatographically purified
Form/Format
Supplied as a dialyzed, lyophilized powder
CAS Number
9001-37-0
Activity
~250u/mg (~300u/mg Protein)
Quality Control
SDS-PAGE
Unit Definition
1 unit oxidizes 1umole of o-dianisidine per minute at 25ºC, pH 6.0.
Preparation and Storage
Stable for 12 months at 2-8 degree C
Stability: Dry preparations are stable for years when stored cold. Solutions are reasonably stable under a variety of conditions.
Stability: Dry preparations are stable for years when stored cold. Solutions are reasonably stable under a variety of conditions.
Testing Data
(EC=1.1.3.4; GOx; Notatin; Glucose oxyhydrase, Beta-D-glucose, Oxygen 1-oxidoreductaase)
Testing Data
(EC=1.1.3.4; GOx; Notatin; Glucose oxyhydrase, Beta-D-glucose, Oxygen 1-oxidoreductaase)
Structure
(Structure of glucose oxidase dimer (dark and light blue) complexed with FAD (salmon) and glycans (aquamarine) from Penicillium amagasakiense)
Structure
(Structure of glucose oxidase dimer (dark and light blue) complexed with FAD (salmon) and glycans (aquamarine) from Penicillium amagasakiense)
Related Product Information for gox enzyme
Since its discovery as an "antibiotic" (shown subsequently to be due to peroxide formation) there has been an interest in glucose oxidase, chiefly because of its utility in glucose estimation. For most clinical work the crude form of the A. niger enzyme has been satisfactory. However, it contains trace amount of polysaccharidases such as amylase, maltase and sucrase which can contribute to falsely high glucose levels. The purified enzyme is free of these traces and is recommended for analytical use in the presence of di-or polysaccharides. Glucose analytical systems employ the enzyme utilizing H2O2 production reacting with Fe(CN)-in the presence of luminol to produce luminescence proportional to the initial glucose concentration.
Product Categories/Family for gox enzyme
NCBI and Uniprot Product Information
Uniprot Description
Catalytic activity: Beta-D-glucose + O2 = D-glucono-1,5-lactone + H2O2.
Cofactor: FAD.
Subunit structure: Homodimer.
Subcellular location: Secreted.
Miscellaneous: This enzyme is widely applied for the determination of glucose in body fluids and in removing residual glucose or oxygen from foods and beverages. Furthermore, glucose oxidase-producing molds such as aspergillus and penicillium species are used for the biological production of gluconic acid.
Sequence similarities: Belongs to the GMC oxidoreductase family.
Similar Products
Product Notes
The Glucose Oxidase, A. niger gox (Catalog #AAA653934) is an Enzyme produced from Aspergillus niger and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Glucose Oxidase, A. niger, Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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