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Western Blot (WB) ((0.1ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Goat CD90/Thy1 Polyclonal Antibody | anti-THY1 antibody

Goat anti-CD90/Thy1 Antibody

Gene Names
THY1; CD90; CDw90
Reactivity
Tested: Human; Expected from sequence similarity: Human, Dog, Pig
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
CD90/Thy1; Polyclonal Antibody; Goat anti-CD90/Thy1 Antibody; THY1; Thy-1 cell surface antigen; CD90; FLJ33325; Thy-1 T-cell antigen; Thy-1 membrane glycoprotein; THY1 antibody; Thy-1 cell surface antigen antibody; CD90 antibody; FLJ33325 antibody; Thy-1 T-cell antigen antibody; Thy-1 membrane glycoprotein antibody; CD90 / Thy1; anti-THY1 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Dog, Pig
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
PEHTYRSRTNFTSKY
Sequence Length
161
Applicable Applications for anti-THY1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx 26kDa band observed in Human Brain (Cerebellum) and Human Tonsil lysates (calculated MW of 17.9kDa according to NP_006279.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3ug/ml.
Immunogen
Peptide with sequence C-PEHTYRSRTNFTSKY, from the internal region of the protein sequence according to NP_006279.2.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.1ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((0.1ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,935 Da
NCBI Official Full Name
thy-1 membrane glycoprotein preproprotein
NCBI Official Synonym Full Names
Thy-1 cell surface antigen
NCBI Official Symbol
THY1
NCBI Official Synonym Symbols
CD90; CDw90
NCBI Protein Information
thy-1 membrane glycoprotein
UniProt Protein Name
Thy-1 membrane glycoprotein
UniProt Gene Name
THY1
UniProt Entry Name
THY1_HUMAN

NCBI Description

This gene encodes a cell surface glycoprotein and member of the immunoglobulin superfamily of proteins. The encoded protein is involved in cell adhesion and cell communication in numerous cell types, but particularly in cells of the immune and nervous systems. The encoded protein is widely used as a marker for hematopoietic stem cells. This gene may function as a tumor suppressor in nasopharyngeal carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

Uniprot Description

THY1: May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

Protein type: Membrane protein, GPI anchor; Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: anchored to external side of plasma membrane; apical plasma membrane; cytosol; dendrite; endoplasmic reticulum; external side of plasma membrane; focal adhesion; growth cone; integral to plasma membrane; lipid raft; myelin sheath; plasma membrane

Molecular Function: GTPase activator activity; integrin binding; protein binding; protein kinase binding

Biological Process: angiogenesis; cell-cell adhesion; cytoskeleton organization and biogenesis; focal adhesion formation; negative regulation of axonogenesis; negative regulation of cell migration; negative regulation of protein kinase activity; negative regulation of T cell receptor signaling pathway; positive regulation of GTPase activity; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of T cell activation; protein amino acid autophosphorylation; retinal cone cell development; T cell receptor signaling pathway

Research Articles on THY1

Similar Products

Product Notes

The THY1 thy1 (Catalog #AAA421750) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-CD90/Thy1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Dog, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's CD90/Thy1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: Approx 26kDa band observed in Human Brain (Cerebellum) and Human Tonsil lysates (calculated MW of 17.9kDa according to NP_006279.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the THY1 thy1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: PEHTYRSRTN FTSKY. It is sometimes possible for the material contained within the vial of "CD90/Thy1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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