Rabbit anti-Human CXCR4-Lo Polyclonal Antibody | anti-CXCR4 antibody

CXCR4-Lo Antibody

Cat. Num. AAA150454

• Gene Names: CXCR4; FB22; HM89; LAP3; LCR1; NPYR; WHIM; CD184; LAP-3; LESTR; NPY3R; NPYRL; HSY3RR; NPYY3R; D2S201E
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: CXCR4-Lo Antibody is purified IgG.
• Synonyms: CXCR4-Lo; Polyclonal Antibody; CXCR4-Lo Antibody; FB22; HM89; LAP3; LCR1; NPYR; WHIM; CD184; LESTR; NPY3R; NPYRL; HSY3RR; NPYY3R; D2S201E; C-X-C chemokine receptor type 4; CXC-R4; chemokine (C-X-C motif) receptor 4; anti-CXCR4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody is specific for the longer isoform of CXCR4.
• Purity/Purification: CXCR4-Lo Antibody is purified IgG.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 356

• Applicable Applications for anti-CXCR4 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: CXCR4-Lo antibody can be used for Western blot at 10 mug/mL. Antibody can also be used for immunocytochemistry starting at 2 mug/mL. For immunofluorescence start at 4 mug/mL.
• Conjugate: Unconjugated
• Immunogen: CXCR4-Lo antibody was raised against a peptide corresponding to nine amino acids near the amino terminus of human CXCR4 isoform a.
• Buffer: CXCR4-Lo Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: CXCR4-Lo antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of CXCR4 in (A) human spleen and (B) human thymus tissue lysate with CXCR4-Lo antibody at 10 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of CXCR4Lo in HeLa cells with CXCR4Lo antibody at 2 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of CXCR4-Lo in Hela cells with CXCR4-Lo antibody at 4 μg/mL.)

Related Product Information for anti-CXCR4 antibody

CXCR4-Lo Antibody: Human immunodeficiency virus (HIV) and related viruses require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CXCR4 is a principal coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells. CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2. The alpha-chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1. CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells. CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV binding sites.

NCBI and Uniprot Product Information

• NCBI GI #: 56790927
• NCBI GeneID: 7852
• NCBI Accession #: NP_001008540
• NCBI GenBank Nucleotide #: NM_001008540.1
• UniProt Accession #: P61073; O60835; P30991; P56438; Q53S69; Q9BXA0; Q9UKN2; B2R5N0
• Molecular Weight: 40,221 Da
• NCBI Official Full Name: C-X-C chemokine receptor type 4 isoform a
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) receptor 4
• NCBI Official Symbol: CXCR4
• NCBI Official Synonym Symbols: FB22; HM89; LAP3; LCR1; NPYR; WHIM; CD184; LAP-3; LESTR; NPY3R; NPYRL; HSY3RR; NPYY3R; D2S201E
• NCBI Protein Information: C-X-C chemokine receptor type 4; fusin; CD184 antigen; SDF-1 receptor; LPS-associated protein 3; neuropeptide Y receptor Y3; seven transmembrane helix receptor; stromal cell-derived factor 1 receptor; lipopolysaccharide-associated protein 3; seven-transmembrane-segment receptor, spleen; leukocyte-derived seven transmembrane domain receptor
• UniProt Protein Name: C-X-C chemokine receptor type 4
• Protein Family: C-X-C chemokine receptor
• UniProt Gene Name: CXCR4
• UniProt Synonym Gene Names: CXC-R4; CXCR-4; LESTR; LAP-3; LPS-associated protein 3; SDF-1 receptor
• UniProt Entry Name: CXCR4_HUMAN

NCBI Description

This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

CXCR4: Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. Acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Involved in hematopoiesis and in cardiac ventricular septum formation. Also plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus. Monomer. Can form dimers. Interacts with CD164. Interacts with HIV-1 surface protein gp120 and Tat. Interacts with ARRB2; the interaction is dependent on the C-terminal phosphorylation of CXCR4 and allows activation of MAPK1 and MAPK3. Interacts with ARRC; the interaction is dependent on the C-terminal phosphorylation of CXCR4 and modulates calcium mobilization. Interacts (via the cytoplasmic C-terminal) with ITCH (via the WW domains I and II); the interaction, enhanced by CXCL12, ubiquitinates CXCR4 and leads to its degradation. Interacts with extracellular ubiquitin. Interacts with human cytomegalovirus/HHV- 5 protein UL78. Expressed in numerous tissues, such as peripheral blood leukocytes, spleen, thymus, spinal cord, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, cerebellum, cerebral cortex and medulla (in microglia as well as in astrocytes), brain microvascular, coronary artery and umbilical cord endothelial cells. Isoform 1 is predominant in all tissues tested. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 2q21

Cellular Component: cell surface; lysosome; cytoplasmic membrane-bound vesicle; early endosome; leading edge; integral to membrane; intercellular junction; growth cone; late endosome; cytoplasm; plasma membrane; cytoplasmic vesicle; external side of plasma membrane

Molecular Function: G-protein coupled receptor activity; viral receptor activity; ubiquitin binding; protein binding; ubiquitin protein ligase binding; cytokine binding; myosin light chain binding; coreceptor activity; actin binding; C-X-C chemokine receptor activity

Biological Process: entry of virus into host cell; regulation of chemotaxis; viral reproduction; neutrophil activation; apoptosis; activation of MAPK activity; response to virus; neuron migration; calcium-mediated signaling; motor axon guidance; regulation of cell migration; germ cell development; patterning of blood vessels; T cell proliferation; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; ameboidal cell migration; myelin maintenance; germ cell migration; response to hypoxia; dendritic cell chemotaxis; entry into host cell; positive regulation of oligodendrocyte differentiation; brain development; inflammatory response

Disease: Whim Syndrome