Rabbit anti-Human, Mouse CSGALNACT1 Polyclonal Antibody | anti-CSGALNACT1 antibody

CSGALNACT1, ID (CSGALNACT1, CHGN, GALNACT1, Chondroitin sulfate N-acetylgalactosaminyltransferase 1, Chondroitin beta-1,4-N-acetylgalactosaminyltransferase 1) (PE)

Cat. Num. AAA6289263

• Gene Names: CSGALNACT1; ChGn; CSGalNAcT-1; beta4GalNAcT
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CSGALNACT1; Polyclonal Antibody; ID (CSGALNACT1; CHGN; GALNACT1; Chondroitin sulfate N-acetylgalactosaminyltransferase 1; Chondroitin beta-1; 4-N-acetylgalactosaminyltransferase 1) (PE); 4-N-acetylgalactosaminyltransferase 1; anti-CSGALNACT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-CSGALNACT1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CSGALNACT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acid 200-230 from the Central region of human CSGALNACT1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CSGALNACT1 Antibody (Center) in mouse NIH-3T3 cell line lysates (35ug/lane). CSGALNACT1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(CSGALNACT1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CSGALNACT1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-CSGALNACT1 antibody

CSGALNACT1 transfers 1,4-N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of glucuronic acid (GlcUA). CSGALNACT1 required for addition of the first GalNAc to the core tetrasaccharide linker and for elongation of chondroitin chains. CSGALNACT1 is important role in chondroitin chain biosynthesis in cartilage.

Product Categories/Family for anti-CSGALNACT1 antibody

Antibodies; Enzymes

References

Landa, I., et al. PLoS Genet. 5 (9), E1000637 (2009). Sakai, K., et al. J. Biol. Chem. 282(6):4152-4161(2007). Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 194473683
• NCBI GeneID: 55790
• NCBI Accession #: NP_001123990.1
• NCBI GenBank Nucleotide #: NM_001130518.1
• UniProt Accession #: Q8TDX6; Q6P9G6; Q8IUF9; Q9NSQ7; Q9NUM9; B2RBE4
• Molecular Weight: 61,294 Da
• NCBI Official Full Name: chondroitin sulfate N-acetylgalactosaminyltransferase 1
• NCBI Official Synonym Full Names: chondroitin sulfate N-acetylgalactosaminyltransferase 1
• NCBI Official Symbol: CSGALNACT1
• NCBI Official Synonym Symbols: ChGn; CSGalNAcT-1; beta4GalNAcT
• NCBI Protein Information: chondroitin sulfate N-acetylgalactosaminyltransferase 1; beta4GalNAcT-1; chondroitin beta1,4 N-acetylgalactosaminyltransferase; chondroitin beta-1,4-N-acetylgalactosaminyltransferase 1
• UniProt Protein Name: Chondroitin sulfate N-acetylgalactosaminyltransferase 1
• Protein Family: Chondroitin sulfate N-acetylgalactosaminyltransferase
• UniProt Gene Name: CSGALNACT1
• UniProt Synonym Gene Names: CHGN; GALNACT1; CsGalNAcT-1
• UniProt Entry Name: CGAT1_HUMAN

Uniprot Description

ChGn: Transfers 1,4-N-acetylgalactosamine (GalNAc) from UDP- GalNAc to the non-reducing end of glucuronic acid (GlcUA). Required for addition of the first GalNAc to the core tetrasaccharide linker and for elongation of chondroitin chains. Important role in chondroitin chain biosynthesis in cartilage. Belongs to the chondroitin N- acetylgalactosaminyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; Membrane protein, integral; Glycan Metabolism - chondroitin sulfate biosynthesis; EC 2.4.1.174

Chromosomal Location of Human Ortholog: 8p21.3

Cellular Component: Golgi membrane; integral to Golgi membrane; intracellular

Molecular Function: glucuronosyltransferase activity; metal ion binding; acetylgalactosaminyltransferase activity; glucuronylgalactosylproteoglycan 4-beta-N-acetylgalactosaminyltransferase activity; peptidoglycan glycosyltransferase activity; glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase activity

Biological Process: nervous system development; anatomical structure morphogenesis; extracellular matrix organization and biogenesis; chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; chondroitin sulfate proteoglycan biosynthetic process; cell recognition; pathogenesis; UDP-N-acetylgalactosamine metabolic process; chondroitin sulfate metabolic process; cell proliferation; UDP-glucuronate metabolic process; heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process; heparin biosynthetic process; cartilage development; proteoglycan biosynthetic process; carbohydrate metabolic process; chondroitin sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process; dermatan sulfate proteoglycan biosynthetic process; endochondral ossification