Chromatin Immunoprecipitation (ChIP)
(N-Myc antibody (pAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 200 ug of chromatin from human cell line chromatin and 20 ug N-Myc antibody. ChIP DNA was sequenced on the Illumina HiSeq and 9.2 million sequence tags were mapped to identify N-Myc binding sites on chromosome 3. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.)
Rabbit anti-Human N-Myc Polyclonal Antibody | anti-N-Myc antibody
N-Myc antibody (pAb)
ChIP-Seq: 10-20ul per ChIP
WB: 1:500-1:2,000 dilution
For optimal results in Western Blot, primary antibody incubations should be performed at 4 degree C.
Chromatin Immunoprecipitation (ChIP)
(N-Myc antibody (pAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 200 ug of chromatin from human cell line chromatin and 20 ug N-Myc antibody. ChIP DNA was sequenced on the Illumina HiSeq and 9.2 million sequence tags were mapped to identify N-Myc binding sites on chromosome 3. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.)
Chromatin Immunoprecipitation (ChIP)
(N-Myc antibody (pAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 200 ug of chromatin from human cell line chromatin and 20 ug N-Myc antibody. ChIP DNA was sequenced on the Illumina HiSeq and 9.2 million sequence tags were mapped to identify N-Myc binding sites on chromosome 3. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.)
Western Blot (WB)
(N-Myc antibody (pAb) tested by Western blot. Nuclear extract (20 ug) of Kelly cells probed with N-Myc antibody at a dilution of 1:1,000)
Western Blot (WB)
(N-Myc antibody (pAb) tested by Western blot. Nuclear extract (20 ug) of Kelly cells probed with N-Myc antibody at a dilution of 1:1,000)
Background: N-Myc (MYCN) is a member of the Myc family of proto-oncogenes. Myc family members play crucial roles in regulating cell proliferation, size and differentiation. N-Myc is required for proper development of the nervous system. Myc family proteins can induce malignant transformation inappropriately modulating gene transcription, leading to unchecked cell proliferation. Misexpression of N-Myc is often associated with pediatric neural cancers such as neuroblastoma, medulloblastoma and retinoblastoma.
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]
Uniprot Description
N-Myc: a proto-oncogenic transcription factor. Plays a role in cell proliferation, apoptosis and in the development of human tumors.
Protein type: Oncoprotein; DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 2p24.3
Cellular Component: nucleus; chromatin
Molecular Function: protein dimerization activity; protein binding; DNA binding; transcription factor activity
Biological Process: regulation of transcription from RNA polymerase II promoter; branching morphogenesis of a tube; negative regulation of astrocyte differentiation; positive regulation of mesenchymal cell proliferation; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; embryonic skeletal morphogenesis; cartilage condensation; lung development
Disease: Feingold Syndrome 1; Tracheoesophageal Fistula With Or Without Esophageal Atresia
Research Articles on N-Myc
Similar Products
Product Notes
The N-Myc mycn (Catalog #AAA388798) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The N-Myc antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's N-Myc can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation-Seq (ChIP-Seq), Western Blot (WB). Applications Validated: ChIP-Seq: 10-20ul per ChIP WB: 1:500-1:2,000 dilution For optimal results in Western Blot, primary antibody incubations should be performed at 4 degree C. Researchers should empirically determine the suitability of the N-Myc mycn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "N-Myc, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
