Western Blot (WB)
(Figure 1. Western blot analysis of SOX18 using anti-SOX18 antibody (MBS1753634).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human Jurkat whole cell lysatesLane 2: human COLO-320 whole cell lysatesLane 3: human Hela whole cell lysatesLane 4: human HepG2 whole cell lysatesLane 5: monkey COS-7 whole cell lysatesLane 6: rat heart tissue lysatesLane 7: rat lung tissue lysatesLane 8: rat liver tissue lysatesLane 9: mouse lung tissue lysatesLane 10: mouse liver tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-SOX18 antigen affinity purified polyclonal antibody (Catalog # MBS1753634) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for SOX18 at approximately 41KD. The expected band size for SOX18 is at 41KD. )
Rabbit SOX18 Polyclonal Antibody | anti-SOX18 antibody
Anti-SOX18 Antibody
ICC/IF: 5ug/ml|Human|
FC/FACS/FCM: 1-3ug/1x106 cells|Human|
Direct ELISA: 0.1-0.5ug/ml|Human|
Western Blot (WB)
(Figure 1. Western blot analysis of SOX18 using anti-SOX18 antibody (MBS1753634).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human Jurkat whole cell lysatesLane 2: human COLO-320 whole cell lysatesLane 3: human Hela whole cell lysatesLane 4: human HepG2 whole cell lysatesLane 5: monkey COS-7 whole cell lysatesLane 6: rat heart tissue lysatesLane 7: rat lung tissue lysatesLane 8: rat liver tissue lysatesLane 9: mouse lung tissue lysatesLane 10: mouse liver tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-SOX18 antigen affinity purified polyclonal antibody (Catalog # MBS1753634) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for SOX18 at approximately 41KD. The expected band size for SOX18 is at 41KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of SOX18 using anti-SOX18 antibody (MBS1753634).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human Jurkat whole cell lysatesLane 2: human COLO-320 whole cell lysatesLane 3: human Hela whole cell lysatesLane 4: human HepG2 whole cell lysatesLane 5: monkey COS-7 whole cell lysatesLane 6: rat heart tissue lysatesLane 7: rat lung tissue lysatesLane 8: rat liver tissue lysatesLane 9: mouse lung tissue lysatesLane 10: mouse liver tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-SOX18 antigen affinity purified polyclonal antibody (Catalog # MBS1753634) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for SOX18 at approximately 41KD. The expected band size for SOX18 is at 41KD. )
Immunofluorescence (IF)
(Figure 2. IF analysis of SOX18 using anti- SOX18 antibody (MBS1753634).SOX18 was detected in immunocytochemical section of HepG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- SOX18 Antibody (MBS1753634) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. )
Immunofluorescence (IF)
(Figure 2. IF analysis of SOX18 using anti- SOX18 antibody (MBS1753634).SOX18 was detected in immunocytochemical section of HepG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- SOX18 Antibody (MBS1753634) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. )
Flow Cytometry (FC/FACS)
(Figure 3. Flow Cytometry analysis of HepG2 cells using anti-SOX18 antibody (MBS1753634).Overlay histogram showing HepG2 cells stained with MBS1753634 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SOX18 Antibody (MBS1753634, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 3. Flow Cytometry analysis of HepG2 cells using anti-SOX18 antibody (MBS1753634).Overlay histogram showing HepG2 cells stained with MBS1753634 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SOX18 Antibody (MBS1753634, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
2. Devriendt, K., Vikkula, M., Irrthum, A., Mattjijs, G., Mertens, A., Fryns, J. -P. Autosomal recessive alopecia and lymphedema. (Abstract) Genet. Counsel. 13: 74-75, 2002.
3. Downes, M., Francois, M., Ferguson, C., Parton, R. G., Koopman, P. Vascular defects in the mouse model of hypotrichosis-lymphedema-telangiectasia syndrome indicate a role for SOX18 in blood vessel maturation. Hum. Molec. Genet. 18: 2839-2850, 2009.
NCBI and Uniprot Product Information
Observed: 44 kDa
NCBI Description
This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. This protein plays a role in hair, blood vessel, and lymphatic vessel development. Mutations in this gene have been associated with recessive and dominant forms of hypotrichosis-lymphedema-telangiectasia. [provided by RefSeq, Jul 2008]
Uniprot Description
SOX18: Binds to the consensus sequence 5'-AACAAAG-3' and is able to trans-activate transcription via this site. Defects in SOX18 are the cause of hypotrichosis- lymphedema-telangiectasia syndrome (HLTS).
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 20q13.33
Cellular Component: nuclear chromatin; nucleus
Molecular Function: protein heterodimerization activity
Biological Process: blood vessel endothelial cell migration; in utero embryonic development; positive regulation of transcription, DNA-dependent; cell maturation; stem cell fate specification; negative regulation of transcription from RNA polymerase II promoter; mRNA transcription from RNA polymerase II promoter; hair cycle process; hair follicle development; vasculature development; lymphangiogenesis; embryonic heart tube development; positive regulation of transcription from RNA polymerase II promoter; heart looping; angiogenesis; vasculogenesis; negative regulation of transcription, DNA-dependent
Disease: Hypotrichosis-lymphedema-telangiectasia Syndrome; Hypotrichosis-lymphedema-telangiectasia-renal Defect Syndrome
Research Articles on SOX18
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Product Notes
The SOX18 sox18 (Catalog #AAA1753634) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SOX18 Antibody reacts with Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SOX18 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA). WB: 0.1-0.25ug/ml|Human, Monkey, Mouse, Rat| ICC/IF: 5ug/ml|Human| FC/FACS/FCM: 1-3ug/1x106 cells|Human| Direct ELISA: 0.1-0.5ug/ml|Human|. Researchers should empirically determine the suitability of the SOX18 sox18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SOX18, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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