Bovine Myoglobin ELISA Kit | Mb elisa kit

Bovine Myoglobin ELISA Kit

Cat. Num. AAA735687

• Gene Names: MB; PVALB; myoglobgin
• Reactivity: Bovine
• Synonyms: Myoglobin; Bovine Myoglobin ELISA Kit; Mb elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve

Related Product Information for Mb elisa kit

Intended Uses: This Myoglobin ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine Myoglobin. This ELISA kit for research use only!

Principle of the Assay||Myoglobin ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Myoglobin antibody and an Myoglobin-HRP conjugate. The assay sample and buffer are incubated together with Myoglobin-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Myoglobin concentration since Myoglobin from samples and Myoglobin-HRP conjugate compete for the anti-Myoglobin antibody binding site. Since the number of sites is limited, as more sites are occupied by Myoglobin from the sample, fewer sites are left to bind Myoglobin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Myoglobin concentration in each sample is interpolated from this standard curve.

Product Categories/Family for Mb elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 44955888
• NCBI GeneID: 4151
• NCBI Accession #: NP_976312.1
• NCBI GenBank Nucleotide #: NM_203378.1
• Molecular Weight: 17,184 Da
• NCBI Official Full Name: myoglobin
• NCBI Official Synonym Full Names: myoglobin
• NCBI Official Symbol: MB
• NCBI Official Synonym Symbols: PVALB; myoglobgin
• NCBI Protein Information: myoglobin
• UniProt Protein Name: Myoglobin
• Protein Family: Myoglobin
• UniProt Gene Name: MB
• UniProt Entry Name: MYG_HUMAN

NCBI Description

This gene encodes a member of the globin superfamily and is expressed in skeletal and cardiac muscles. The encoded protein is a haemoprotein contributing to intracellular oxygen storage and transcellular facilitated diffusion of oxygen. At least three alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

MB: Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles. Belongs to the globin family.

Protein type: Carrier

Chromosomal Location of Human Ortholog: 22q13.1

Molecular Function: iron ion binding; heme binding; oxygen binding; oxygen transporter activity

Biological Process: slow-twitch skeletal muscle fiber contraction; response to hydrogen peroxide; oxygen transport; heart development; response to hypoxia; brown fat cell differentiation; response to hormone stimulus; enucleate erythrocyte differentiation